Analysis of HPV-16 E6-related gene expression using cDNA microarray.
- Author:
Jin MYEONG
1
;
Sun Young RHA
;
Myoung Jin LEE
;
Soo Jong UM
;
Sung Eun NAMKOONG
;
Jong Sup PARK
Author Information
1. Department of Obstetrics and Gynecology, Catholic University Medical College, Korea.
- Publication Type:Original Article
- Keywords:
Human papillomavirus;
cDNA microarray;
Cellular genes;
HPV-16 E6
- MeSH:
Adenocarcinoma;
Apoptosis;
Cadherins;
Carrier Proteins;
Cell Adhesion;
Cell Cycle;
Cell Line;
Centers for Disease Control and Prevention (U.S.);
Chromosome Segregation;
Colon;
Colonic Neoplasms;
Desmoglein 1;
DNA, Complementary*;
Endothelin-2;
Expressed Sequence Tags;
Gene Expression*;
GTPase-Activating Proteins;
Hand Strength;
Human papillomavirus 16*;
Humans;
Lung;
Metabolism;
Oligonucleotide Array Sequence Analysis*;
Oncogenes;
Oxidation-Reduction;
Protein Transport;
Receptor, Epidermal Growth Factor;
Signal Transduction;
Transcription Factors;
Transferases
- From:Korean Journal of Obstetrics and Gynecology
2002;45(12):2280-2288
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To examine the effect of HPV-16 E6 expression on the transcription of cellular genes, we used cDNA microarray in HPV-16 E6 transfected stable cancer cell lines. METHODS: Using cDNA microarray consisting of 1,024 genes, we have performed a systematic characterization of gene expression in A549E6 human lung adenocarcinoma and RC10.1 human colon adenocarcinoma cell lines stably expressing HPV-16 E6 gene. The up-regulated and down-regulated genes were classified into the different functional categories; oncogenes, apoptosis, cell cycle, signal transduction, gene regulation, immune response, cell adhesion, protein transport, metabolism, redox control and angiogenesis. RESULTS: Among 1,024 known genes and ESTs (expressed sequence tags) tested, we found 27 up- regulated and 43 down-regulated genes in A549E6 (HPV-16 E6) compared to A549. The major up-regulated genes were as follows. GTPase-activating protein Rho 4, transcription factor D2, IKAROS, integrin-alpha 6, cadherin 11, ephrin-beta 2, RAN binding protein 2, branched-chain amino transferase 2. The major down-regulated genes were as follows. K-ras 2, CDC (cell division cycle) 37, CDC16, CDC7L1, IRF3, interferon-gamma-inducible protein 30, cadherin 6, desmoglein 1, desmocollin 2, endothelin 2. Also, we found 48 up-regulated and 34 down-regulated genes in RC10.1 (HPV-16 E6) compared to RKO. The major up-regulated genes were as follows. Colon cancer familial nonpolyposis type 1 (COCA 1), Bcl 2, jagged 1, MAP2K6, E2F1, ephrin receptor-beta 2, ephrin-beta 2, desmoglein 1, transforming growth factor-beta 3. The major down-regulated genes were as follows. KIT, Rad51C, Bcl 2 antagonist killer 1, STAT 4, epidermal growth factor receptor, high mobility group protein 2, cadherin 11, cadherin 12, cadherin 3, integrin-alpha 1, intergrin-alpha 8, chromosome segregation 1-like. CONCLUSION: Various expression patterns of cellular genes by HPV-16 E6 could be wholy grasped and classified into different functional groups using both cell line system stably expressed HPV-16 E6 and cDNA microarray analysis. These analysis methods must be helpful to understand multiple effects of a specific gene on cellular genes in a short period.
