Differentiated Muscle-derived Stem Cells Attenuate Intimal Hyperplasia after Carotid Balloon Injury in Rat.
10.4174/jkss.2010.79.Suppl1.S7
- Author:
In Mok JUNG
;
So Rhee HAN
;
Keum Hee CHOI
;
Yujin KWON
;
Taeseung LEE
;
Seung Kee MIN
;
Yang Jin PARK
;
Jung Kee CHUNG
;
Jongwon HA
;
Sang Joon KIM
- Publication Type:Original Article
- Keywords:
Intimal hyperplasia;
Stem cell therapy;
Muscle derived stem cell
- MeSH:
Animals;
Antigens, CD;
Arteries;
Cadherins;
Carotid Arteries;
Catheters;
Endothelial Cells;
Humans;
Hyperplasia;
Male;
Muscles;
Rats;
Stem Cells;
Vascular Endothelial Growth Factor A;
Viruses
- From:Journal of the Korean Surgical Society
2010;79(Suppl 1):S7-S15
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Although progenitor cells may contribute to intimal hyperplasia (IH) after arterial injury, positive contribution of IH is variable with type of injury or cells. This study was designed to examine whether differentiated muscle derived stem cells (MDSC) attenuate IH in rat. METHODS: MDSCs were retrieved using preplate techniques from rat calf muscle and MDSCs (preplate 6th culture fraction, pp6) were exposed to VEGF (50 ng/ml) for endothelial differentiation prior to injection. Male rats were divided into two groups (cell treated vs. control) and underwent carotid balloon injury with 2-Fr catheter. The virus containing Green fluorescent protein (GFP) gene was transfected into cells for monitoring. Cells (5x10(6)) were indwelled into carotid artery for 30 minutes after injury and then blood flow was restored. Arteries were harvested at various intervals (1, 2 and 4 weeks) after injury. The intima to media thickness ratio (IMTR) was calculated with morphometric analysis. RESULTS: Endothelial surface markers such as VE-CADHERIN were strongly expressed on differentiated MDSCs. At 4 weeks after injury, IH was predominantly observed in control group compared to cell treated group. The intensity of GFP was strongly observed at 1 week and declined at 4 weeks in carotid artery wall at MDSC group. CD31(+) endothelial cells were observed at MDSC group compared to control. The mean IMTR in cell treated groups were significantly lower than control at 2 weeks (P=0.005) and 4 weeks (P< or =0.001). CONCLUSION: Our study demonstrates that MDSCs therapy promotes re-endothelialization and leads to attenuation of IH after balloon injury in rat.
