The Molecular Mechanism of Baicalin on RANKL-induced Osteoclastogenesis in RAW264.7 Cells.
- Author:
Seon Yle KO
1
Author Information
1. Department of Oral biochemistry and Institute of Dental Science, Dankook University, Korea. seonyleko@dankook.ac.kr
- Publication Type:Original Article
- Keywords:
Baicalin;
Osteoclastogenesis;
MAPK;
NFATc1
- MeSH:
Acid Phosphatase;
Carbonic Anhydrase II;
Cathepsin K;
Flavonoids;
Isoenzymes;
NF-kappa B;
NFATC Transcription Factors;
Osteoclasts;
RNA, Messenger;
Scutellaria baicalensis;
Transcription Factors
- From:International Journal of Oral Biology
2013;38(2):67-72
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study examined the anti-osteoclastogenic effects of baicalin on receptor activator of NF-kB ligand (RANKL)-induced RAW264.7 cells. Baicalin is a flavonoid that is produced by Scutellaria baicalensis and is known to have multiple biological properties, including antibacterial, anti-inflammatory and analgesic effects. The effects of baicalin on osteoclasts were examined by measuring 1) cell viability; 2) the formation of tartrate-resistant acid phosphatase (TRAP) (+) multinucleated cells; 3) RANK/RANKL signaling pathways and 4) mRNA levels of osteoclast-associated genes. Baicalin inhibited the formation of RANKL-stimulated TRAP (+) multinucleated cells and also suppressed the RANKL-stimulated activation of p-38, ERK, cSrc and AKT signaling. Baicalin also inhibited the RANKL-stimulated degradation of IkappaB in RAW264.7 cells. In addition, the RANKL-stimulated induction of NFATc1 transcription factors was found to be abrogated by this flavonoid. Baicalin was further found to decrease the mRNA expression of osteoclast-associated genes, including carbonic anhydrase II, TRAP and cathepsin K in the RAW264.7 cells. Our data thus demonstrate that baicalin inhibits osteoclastogenesis by inhibiting the RANKL-induced activation of signaling molecules and transcription factors in osteoclast precursors.
