Identification of HLA-B*5001 in Korean Population.
- Author:
Han Chul KIM
1
;
Il Ho YANG
;
Su Yeon KIM
;
So Yang PARK
;
Seo Young CHUNG
;
Ha Jeung YU
;
Hoon HAN
;
Hye Jung LEE
Author Information
1. Department of Pediatrics, St' Francisco Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Umbilical cord blood;
ARMS-PCR;
HLA-B50
- MeSH:
Alleles;
Bone Marrow Transplantation;
DNA;
Donor Selection;
Fetal Blood;
Genotype;
HLA-B Antigens;
Homozygote;
Leukocytes
- From:Korean Journal of Pediatric Hematology-Oncology
2001;8(2):323-330
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: HLA (human leukocyte antigen)-class I genes are highly polymorphic, play many roles in organ and bone marrow transplantation. HLA-B is the most polymorphic class I locus with 414 alleles. HLA-class I typing, which is based on serologic method, has been used until recently. The development of molecular biological techniques make it possible to define the genotypes of HLA genes. METHODS: Analyses of HLA-B genotyping on 1,000 UCB (Umbilical Cord Blood) samples which were considered to be HLA-B homozygote or blank were performed by ARMS-PCR (Amplification Refractory Mutation System-PCR) method and direct sequencing. RESULTS: We could identify HLA-B*5001 which was known to be absent in Koreans. CONCLUSION: It is strongly suggested that HLA-B homozygote should be confirmed to the DNA level especially in cases of donor selection for the unrelated bone marrow transplantation.
