Effects of White Radish (Raphanus sativus) Enzyme Extract on Hepatotoxicity.
- Author:
Sang Wha LEE
1
;
Kwang Mo YANG
;
Jung Ki KIM
;
Byung Hyouk NAM
;
Chang Min LEE
;
Min Ho JEONG
;
Su Yeong SEO
;
Gi Yong KIM
;
Wol Soon JO
Author Information
1. Department of Research Center, Dong Nam Institute of Radiological & Medical Sciences, Busan 619-953, Korea. sailorjo@dirams.re.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Raphanus sativus;
Radish;
Enzyme extract;
Tacrine;
CCl4;
Hepatoprotection
- MeSH:
Animals;
Carbon Tetrachloride;
Hep G2 Cells;
Humans;
Inhibitory Concentration 50;
Liver;
Medicine, Traditional;
Raphanus;
Rats;
Silymarin;
Tacrine
- From:Toxicological Research
2012;28(3):165-172
- CountryRepublic of Korea
- Language:English
-
Abstract:
Raphanus sativus (Cruciferaceae), commonly known as radish is widely available throughout the world. From antiquity it has been used in folk medicine as a natural drug against many toxicants. The present study was designed to evaluate the hepatoprotective activity of radish (Raphanus sativus) enzyme extract (REE) in vitro and in vivo test. The IC50 values of REE in human liver derived HepG2 cells was over 5,000 microg/ml in tested maximum concentration. The effect of REE to protect tacrine-induced cytotoxicity in HepG2 cells was evaluated by MTT assay. REE showed their hepatoprotective activities on tacrine-induced cytotoxicity and the EC50 value was 1,250 microg/ml. Silymarin, an antihepatotoxic agent used as a positive control exhibited 59.7% hepatoprotective activitiy at 100 microg/ml. Moreover, we tested the effect of REE on carbon tetrachloride (CCl4)-induced liver toxicity in rats. REE at dose of 50 and 100 mg/kg and silymarin at dose of 50 mg/kg were orally administered to CCl4-treated rats. The results showed that REE and silymarin significantly reduced the elevated levels of serum enzyme markers induced by CCl4. The biochemical data were supported by evaluation with liver histopathology. These findings suggest that REE, can significantly diminish hepatic damage by toxic agent such as tacrine or CCl4.
