Protective Effects of Houttuynia cordata Thunb. on Gentamicin-induced Oxidative Stress and Nephrotoxicity in Rats.
- Author:
Changgeun KANG
1
;
Hyungkyoung LEE
;
Do Yun HAH
;
Jung Ho HEO
;
Chung Hui KIM
;
Euikyung KIM
;
Jong Shu KIM
Author Information
1. Department of Pharmacology & Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea. jskim@gnu.ac.kr
- Publication Type:Original Article
- Keywords:
Houttuynia cordata Thunb;
Gentamicin sulfate;
Oxidative stress;
Nephrotoxicity;
Antioxidant;
Reactive oxygen species (ROS)
- MeSH:
Animals;
Blood Urea Nitrogen;
Catalase;
Cats;
Creatinine;
Drug Combinations;
Gentamicins;
Glutathione;
Glycerides;
Houttuynia;
Kidney;
Malondialdehyde;
Monoterpenes;
Oxidative Stress;
Rats;
Rats, Sprague-Dawley;
Superoxide Dismutase
- From:Toxicological Research
2013;29(1):61-67
- CountryRepublic of Korea
- Language:English
-
Abstract:
Development of a therapy providing protection from, or reversing gentamicin-sulfate (GS)-induced oxidative stress and nephrotoxicity would be of great clinical significance. The present study was designed to investigate the protective effects of Houttuynia cordata Thunb. (HC) against gentamicin sulfate-induced renal damage in rats. Twenty-eight Sprague-Dawley rats were divided into 4 equal groups as follows: group 1, control; group 2, GS 100 mg/kg/d, intraperitoneal (i.p.) injection; group 3, GS 100 mg/kg/d, i.p. + HC 500 mg/kg/d, oral; and group 4, GS 100 mg/kg/d i.p. + HC 1000 mg/kg/d, oral administration). Treatments were administered once daily for 12 d. After 12 d, biochemical and histopathological analyses were conducted to evaluate oxidative stress and renal nephrotoxicity. Serum levels of creatinine, malondialdehyde (MDA), and blood urea nitrogen (BUN), together with renal levels of MDA, glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) were quantified to evaluate antioxidant activity. Animals treated with GS alone showed a significant increase in serum levels of creatinine, BUN, and MDA, with decreased renal levels of GSH, SOD, and CAT. Treatment of rats with HC showed significant improvement in renal function, presumably as a result of decreased biochemical indices and oxidative stress parameters associated with GS-induced nephrotoxicity. Histopathological examination of the rat kidneys confirmed these observations. Therefore, the novel natural antioxidant HC may protect against GS-induced nephrotoxicity and oxidative stress in rats.
