Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies.
10.3346/jkms.2007.22.3.405
- Author:
Se Lim KIM
1
;
Hwan Jeong JEONG
;
Eun Mi KIM
;
Chang Moon LEE
;
Tae Hyoung KWON
;
Myung Hee SOHN
Author Information
1. Department of Nuclear Medicine, Chonbuk National University Hospital, 634-18 Geumam-2 dong, Duckjin-gu, Jeonju, Korea. jayjeong@chonbuk.ac.kr
- Publication Type:Original Article ; In Vitro ; Research Support, Non-U.S. Gov't
- Keywords:
Polyethylene Glycol;
Folate Receptor;
Gamma Camera Imaging
- MeSH:
Animals;
Carrier Proteins/*metabolism;
Cell Line, Tumor;
Female;
Fluorescein-5-isothiocyanate/pharmacology;
Folic Acid/*metabolism;
Humans;
Image Processing, Computer-Assisted;
Mice;
Mice, Nude;
Microscopy, Fluorescence;
Neoplasm Transplantation;
Polyethylene Glycols/*chemistry;
Receptors, Cell Surface/*metabolism;
Technetium/*chemistry;
Time Factors
- From:Journal of Korean Medical Science
2007;22(3):405-411
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to ascertain the folate receptor (FR) targetability by an in vitro study and to acquire FR-targeted images in vivo models by using synthetic folate conjugates. PEG-folate was synthesized and labeled with (99m)Tc and fluorescein isothiocynate (FITC). Cell uptake studies were carried out in KB cells (FR-positive) and A549 cells (FR-negative) using FITC- and the (99m)Tc-labeled conjugates. The radiolabeled conjugate was intravenously injected to KB tumor xenografted mice. After it was injected, gamma images were recorded at 30 min, 1, 2, 3 and 4 hr. Cell uptake studies showed a difference between the KB cells and the A549 cells by flow cytometry analysis and gamma counting. On in vivo images, the tumor-tonormal muscle ratio was greater than 4. It ascertained that the PEG-folate conjugate specifically binds to the FR expressed on tumor cells in vitro. Moreover, it was possible to acquire the FR-targeted gamma images using PEG-folate conjugates in tumor models.
