The Effect of Fibronectin, Hyaluronic Acid and Growth Factors on the Wound Healing of Cultured Rabbit Corneal Endothelial Cells.
- Author:
Ki San KIM
1
;
Duk Kee HAHN
Author Information
1. Department of Ophthalmology, Yeungnam University School of Medicine, Taegu, Korea.
- Publication Type:Original Article
- Keywords:
Corneal endothelial cell;
DNA synthesis;
Fibronectin;
Growth factor;
Hyaluronic acid
- MeSH:
Cell Movement;
Cell Proliferation;
Descemet Membrane;
DNA;
Endothelial Cells*;
Epidermal Growth Factor;
Fibroblast Growth Factors;
Fibronectins*;
Hyaluronic Acid*;
Intercellular Signaling Peptides and Proteins*;
Silicones;
Transforming Growth Factor beta;
Wound Healing*;
Wounds and Injuries*
- From:Journal of the Korean Ophthalmological Society
1992;33(5):436-456
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To investigate the effects of fibronectin (FN), hyaluronic acid (HA), epidermal growth factor (EGF), fibroblast growth factor (FGF), and transforming growth factor-beta(TGF-beta) on the healing of the corneal endothelial cells, the rabbit corneal endothelial cells were cultured from the outgrowth of Descemet's membrane explants. After making a wound at the center of the monolayer of confluent corneal endothelial cells using a rotating silicone tip, three different concentrations of testing agents were added. Then, the wound healing area and healing rate were measured. In addition, the effects of agents on the DNA synthesis by corneal endothelial cells were determined by measuring the incorporation of 3H-thymidine into cold acid-insoluble material. FN and EGF increased the wound healing area at all concentrations tested, and HA only at 1.0mg/ml showed a stimulatory effect. However, FGF showed little effect at all concentrations. TGF-beta promoted wound healing at 1.0 and 10.0 ng/ml. The combined treatment of FN and HA caused a more potent effect on corneal endothelial wound healing than single treatment. Neither the combined treatment of EGF and TGF-beta, nor the combined treatment of FGF and TGF-beta caused any significant effect. Wound healing rate was highest during the first 12 hours in all agents. EGF stimulated DNA synthesis while other agents studied did not, and TGF-beta suppressed EGF-induced DNA synthesis. These results indicate that FN, HA and TGF-beta stimulate the endothelial wound healing by probably enhancing cell migration, and EGF by enhancing cell migration and cell proliferation.
