The Basic Studies on the Application of Galectin - 3 as a Target Protein for Delivering Anticancer Agents.
- Author:
Hee Jong WOO
;
Hong Gu JOO
- Publication Type:Original Article
- Keywords:
galectin-3;
gastric cancer;
immunotoxin;
doxorubicin
- MeSH:
Antineoplastic Agents*;
Cell Line;
Doxorubicin;
Flow Cytometry;
Galectin 3;
Galectins*;
Immunoconjugates;
Immunotoxins;
Staphylococcal Protein A;
Stomach Neoplasms
- From:Korean Journal of Immunology
1997;19(1):83-90
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Gastric cancer cells express large amounts of galectin-3 on the cell surface. This fact may provide the possibility to use galectin-3 protein as a surface target for delivering cytotoxic anticancer agents. To investigate the possibility of application of galectin-3 protein as a target protein in delivering cytotoxic anticancer agents, we synthesized doxorubicin immunoconjugate by using maleimidocaproic acid and conjugated doxorubicin immunoconjugate to anti-galectin-3 mAb. The anticancer effect of immunotoxin was assayed on NIH3T3, AGS and KATO III cell lines. The anticancer effect of immunotoxin on AGS cell line is highest and that of KATO III is higher than that of NIH3T3. This results relate to that of flow cytometry analysis previously shown and indicate that galectin-3 protein can be used as a target protein on the surface of gastric cancer cell for delivering cytotoxic anticancer agents.
