Development of Monoclonal Antibodies Recognizing Human Peripheral Blood T Lymphocytes Cytoplasmic Proteins Induced upon Activation.
- Author:
Yang Ja CHO
;
Yong Hoon CHUNG
;
Yong CHOI
;
Yong Sik KIM
- Publication Type:Original Article
- Keywords:
monoclonal antibody;
activated T cell antigen;
heat shock proteins
- MeSH:
Antibodies, Monoclonal*;
Antigen-Presenting Cells;
Antigens, Surface;
Apoptosis;
Cell Proliferation;
Cytoplasm*;
Heat-Shock Proteins;
Heat-Shock Response;
Humans*;
T-Lymphocytes*
- From:Korean Journal of Immunology
1997;19(1):145-156
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Antigen-specific T cell activation requires interaction of the T cell with specialized antigen-presenting cells. Signaling through the TCR is necessary but not sufficient to induce antigen-specific T cell activation and cytokine secretion. This first signal, termed signal 1, is both antigen-specific and MHC-restricted. Signal 2, which is neither antigen-specific nor MHC-restricted, is necessary to induce cytokine secretion, cellular proliferation, and effector function. Recently immunological studies in T cell activation area are mainly focused on biological and molecular biological characterization of TCR/CD3 complex and accessary molecules providing costimulatory signal (signal 2). If signal 2 is not delivered, T cell enter a state of long term un-responsiveness to specific antigen-termed anergy. Monoclonal antibody technique has been especially involved in recognizing novel inducible cell surface antigens on T cell activation. This study was aimed to develop monoclonal antibodies recognizing novel cytoplasmic proteins present in activated T cells. We make 6 monoclones involved in changing pattern of T cell activated cytoplasmic proteins. Using these 6 monoclonal antibodies analyze to find novel molecules involved in T cell activation associated response, apoptosis, and/or heat shock response of the T cells in early T cell activation.
