Anti-adhesive Effect of Poloxamer/Alginate/CaCl2 Mixture in the Rat Model.
- Author:
Sung Won KWON
1
;
Se Hwan LIM
;
Young Woo LEE
;
Yun Gee LEE
;
Bo Young CHU
;
Jin Ho LEE
;
Young Moo LEE
Author Information
1. Department of Surgery, Pochon CHA University College of Medicine, Seongnam, Korea. wizard95@cha.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Adhesion prevention;
Poloxamer;
Alginate;
Degradation;
Animal model
- MeSH:
Abdominal Wall;
Animals;
Cecum;
Inflammation;
Membranes;
Models, Animal*;
Poloxamer;
Rats*;
Sodium;
Tissue Adhesions
- From:Journal of the Korean Surgical Society
2006;71(4):280-287
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The occurrence of post-surgical adhesion is still a major cause of postoperative morbidity due to the lack of satisfactory treatment or prophylaxis. Several adhesion barriers have been developed in the form of solutions or membrane in an attempt to solve these problems. However both types of tissue barriers have some limitations in their practical applications. In order to overcome these problems, a temperature-sensitive Poloxamer/Alginate/CaCl2 mixture was prepared as an adhesion barrier. With this material, toxicity, inflammation and the adhesion prevention effect was evaluated in an animal model. METHODS: The sol-gel transition behavior was measured using a viscometer. An in vitro gel stability test and an in vivo degradation test was performed. The anti-adhesion effect was evaluated using a cecal-abdominal wall abrasion model. The denuded cecum was coated with Poloxamer/ Alginate/CaCl2 mixture, GUARDIX-SL (positive control group) or neither (negative control group) and apposed to the abdominal wall (each n=14). One week after surgery, the level of adhesion was graded from zero to three using a whole-number system. RESULTS: The LCST of the poloxamer/sodium alginate mixture was 25 degrees C. The gel stability of Poloxamer was improved by adding mild cross-linked sodium Alginate/CaCl2 mixture. The adhesion grade and area were significantly lower in the experimental group than in the control. CONCLUSION: The anti-adhesive effect of the Poloxamer/Alginate/CaCl2 mixture was comparable to the previously- developed solution type barrier and all the materials had degraded within 21 days. From these results, Poloxamer/ Alginate/CaCl2 mixture is a good candidate for use as a coatable or injectable tissue adhesion barrier.
