Influence of Pathogen Inactivation on the Quality of Platelet Rich Plasma Derived Platelets.
- Author:
So Yong KWON
1
;
Jae Won KANG
;
Youn Jung CHO
;
Nam Sun CHO
;
Gy Youl NAM
;
Yoon Hwan CHANG
;
Tae Hee HAN
;
Sang Won LEE
Author Information
1. Blood Transfusion Research Institute, Korean Red Cross, Seoul, Korea. sykwon@redcross.or.kr
- Publication Type:Original Article
- Keywords:
Pathogen inactivation;
PRP-PLT;
Quality
- MeSH:
Annexin A5;
Apoptosis;
Blood Platelets;
Humans;
Hydrogen-Ion Concentration;
Organothiophosphorus Compounds;
P-Selectin;
Plasma;
Platelet-Rich Plasma;
Quality Control;
Tissue Donors;
Surveys and Questionnaires
- From:Korean Journal of Blood Transfusion
2013;24(2):128-139
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Pathogen inactivation (PI) is a proactive approach to overcome the limitations of the current testing system and donor questionnaires. Effect of PI on non-leukoreduced platelet rich plasma derived platelets (PRP-PLTs) suspended in plasma has not yet been evaluated. This study was conducted in order to evaluate the effect of PI on the quality of non-leukoreduced PRP-PLTs suspended in plasma. METHODS: PRP-PLTs treated with the Mirasol PRT System and the Intercept Blood System were tested for PLT count, blood gas, PLT activation, and apoptosis on days 3, 5, and 7 of storage. RESULTS: PLT number showed a decrease after PI. No difference in pH was observed until day 5. At day 7, PLTs treated with Mirasol had a lower pH value (6.5), however, it satisfied the quality control criteria. PLTs treated with Mirasol had a lower pO2 compared to pre-inactivation PLTs. pO2 during storage differed significantly between the two PI groups. pCO2 showed a decrease after inactivation and both groups showed a significant difference, compared with the control. PLTs treated with Mirasol had increased P-selectin expression after inactivation; however, difference of P-selectin during storage was not significant compared to the control. P-selectin of PLTs treated with Intercept was significantly different compared to those treated with Mirasol and control. Annexin V showed an increase after inactivation in Mirasol treated PLTs and difference during storage was significant compared to control and Intercept. CONCLUSION: As both PI systems showed satisfactory pH values, the criteria showing a high correlation with in vivo PLT viability and generally applied to monitor quality of PLTs, quality of PRP-PLTs after PI appears not to be negatively affected.
