Human Platelet Antigen Genotyping Using a Multiplex Single-Base Primer Extension Reaction in Koreans.
- Author:
Yun Ji HONG
1
;
Ho Eun CHANG
;
Yousun CHUNG
;
Hwa Jeen LEE
;
Jungwon HYUN
;
Sang Mee HWANG
;
Taek Soo KIM
;
Kyoung Un PARK
;
Junghan SONG
;
Kyou Sup HAN
Author Information
1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. m91w95@dreamwiz.com
- Publication Type:Original Article
- Keywords:
Human platelet antigen;
HPA genotyping;
Multiplex single-base primer extension reaction
- MeSH:
Antigens, Human Platelet;
Blood Platelets;
Genotype;
Humans;
Membrane Glycoproteins;
Polymorphism, Single Nucleotide;
Prevalence;
Purpura;
Purpura, Thrombocytopenic;
Thrombocytopenia, Neonatal Alloimmune;
Tissue Donors
- From:Korean Journal of Blood Transfusion
2013;24(2):147-154
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Alloimmunization of human platelet antigens (HPA) is associated with clinically significant disease, such as platelet refractoriness, neonatal alloimmune thrombocytopenia, or posttransfusion purpura. It is determined by single nucleotide polymorphism of genes for platelet membrane glycoprotein. To date, approximately 27 HPAs have been discovered, and their frequencies differ depending on ethnicity and country. METHODS: We conducted an investigation of prevalence of HPA in the Korean population using a multiplex single-base primer extension reaction (SNaPshot). With 84 specimens from healthy donors, HPA genotyping was performed on 11 different HPAs, including HPA-1, -2, -3, -4, -5, -6, -7, -8, -9, -13, and -15. RESULTS: A total of 90 blood samples were genotyped. The genotype frequencies of HPA were as follows: HPA-1a/1a: 100.0%, -2a/2a: 83.3%, -2a/2b: 14.3%, -2b/2b: 2.4%, -3a/3a: 39.3%, -3a/3b: 52.4%, -3b/3b: 8.3%, -4a/4a: 100.0%, -5a/5a: 95.2%, -5a/5b: 4.8%, -6a/6a: 94.0%, -6a/6b: 6.0%, -7a/7a: 100.0%, -8a/8a: 100.0%, -9a/9a: 97.6%, -9a/9b: 2.4%, -13a/13a: 100.0%, -15a/15a: 23.8%, -15a/15b: 51.2%, and -15b/15b: 25.0%. CONCLUSION: The SNaPshot assay was employed for detection of SNPs in various clinically significant HPA genes. In addition to well-known frequencies of previously reported HPA-1 to -8, this study showed frequencies of HPA-9, -13, and -15 in Koreans for the first time. The SNaPshot technique might be suitable for use in actual clinical testing in patients with platelet alloimmunization.
