Analysis of Xenotropic Murine Leukemia Virus-Related Virus (XMRV) in Korean Blood Donors in a Medical Center.
- Author:
Ho Eun CHANG
1
;
Yun Ji HONG
;
Sang Mee HWANG
;
Taek Soo KIM
;
Woo Kyung BAE
;
Kyoung Un PARK
;
Junghan SONG
;
Kyou Sup HAN
Author Information
1. Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seongnam, Korea. m91w95@dreamwiz.com
- Publication Type:Original Article
- Keywords:
Blood donors;
XMRV;
Real-time polymerase chain reaction
- MeSH:
Animals;
Blood Donors;
DNA;
Fatigue Syndrome, Chronic;
Fibroblasts;
Freezing;
Genes, env;
Genes, gag;
Genome;
Humans;
Mice;
Real-Time Polymerase Chain Reaction;
Xenotropic murine leukemia virus-related virus
- From:Korean Journal of Blood Transfusion
2013;24(2):155-160
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Xenotropic murine leukemia virus-related virus (XMRV) has been detected in peripheral blood mononuclear cells (PBMNs), therefore, it has been regarded as being infectious and transmittable by transfusion. Thus, we attempted to detect XMRV in blood samples in order to confirm the absence of XMRV from blood donors. METHODS: We achieved 165 blood donors and four chronic fatigue syndrome (CFS) patients. We performed real-time polymerase chain reaction using the LightCycler 480 (Roche, Penzberg, Germany) for the gag and env genes of the XMRV genome. DNA was extracted from peripheral blood samples. We used Uracil-N-Glycosylase in order to prevent contamination and DNA extracted from mouse embryonic fibroblasts (MEF) for amplification control. RESULTS: No XMRV was detected in any of the blood donors in both the gag and env genes. In four CFS patients, amplification was not detected in the gag gene. In two of four CFS patients, amplifications were detected and the melting temperature was in agreement with that of MEF control in the env gene. CONCLUSION: Although XMRV was not present in blood samples from blood donors, this is the first report on XMRV in Korean blood donors. We confirmed the absence of XMRV in Korean blood donors, the same as studies reported in other countries.
