The neuroprotective effect of transforming growth factor-beta1 via anti-apoptosis on hypoxic-ischemic brain injury in neonatal rats.
- Author:
Hae Min CHUNG
1
;
Eun Jin CHOI
;
Eok Su SEO
;
Woo Taek KIM
Author Information
1. Department of Pediatrics, School of Medicine, Catholic University of Daegu, Daegu, Korea. wootykim@cu.ac.kr
- Publication Type:Original Article ; In Vitro
- Keywords:
TGF-beta1;
Hypoxic-ischemic;
Brain;
Apoptosis;
Neonatal rat
- MeSH:
Animals;
Anoxia;
Apoptosis;
Blotting, Western;
Brain;
Brain Injuries;
Brain Ischemia;
Caspase 3;
Cell Culture Techniques;
Incubators;
Neurons;
Neuroprotective Agents;
Rats;
Real-Time Polymerase Chain Reaction;
Transforming Growth Factor beta1
- From:Korean Journal of Perinatology
2008;19(1):42-53
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: TGF-beta1 is an important neuronal survival factor to neurons from damage induced by cerebral ischemia. We examined whether treatment with the TGF-beta1 has neuroprotective effects on HI brain injury in neonatal rats using Rice-Vannucci model (in vivo) and in rat brain cortical cell culture induced by hypoxia (in vitro). METHODS: Seven-day-old Sprague-Dawley (SD) rat pups were subjected to left carotid occlusion followed by 2 hour of hypoxic exposure. At the before and after 30 minutes of HI, the animals were injected intracerebrally with TGF-beta1 0.5 ng/kg. In addition, brain cortical cell culture model using pregnant SD rats for 19 days were experimented and induced for hypoxia cell injury. The cell were treated with TGF-beta1 1 ng/mL, 5 ng/mL and 10 ng/mL separately. and incubated in 1% O2 incubator. Apoptosis was measured in the injured hemispheres 7 days after the HI insults using western blot for pro-apoptotic marker-bax, caspase-3 and anti-apoptotic marker-Bcl-2. RESULTS: In western blot and real-time PCR showed Caspase-3, Bax and Bax/Bcl-2 levels was reduced and Bcl-2 level was increased in vivo. In brain cortical cell culture, Bcl-2 expression was greater in the group with low dose of TGF-beta1 (1 ng/mL) in western blot. CONCLUSION: This study thus suggests that the neuroprotective role of TGF-beta1 against HI brain injury is mediated through an anti-apoptotic effect, which offers the possibility of TGF-beta1 application for the treatment of neonatal HI encephalopathy.
