Anticancer Effects of IP6 in a Human Colon Carcinoma Cell Line in Nude Mice Xenografts.
10.3393/jksc.2010.26.2.93
- Author:
Chan Dong KIM
1
;
Jeong Eun LEE
;
Ryung Ah LEE
;
Kwang Ho KIM
Author Information
1. Department of Surgery and Institute for Medical Science, Ewha Womans University School of Medicine, Seoul, Korea. ralee@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
CInositol hexaphosphate (IP6);
Xenografts;
Colon cancer
- MeSH:
Animals;
Body Weight;
Cell Line;
Edible Grain;
Colon;
Colonic Neoplasms;
Diet;
HT29 Cells;
Humans;
Mice;
Mice, Nude;
Phytic Acid;
Transplantation, Heterologous;
Tumor Burden
- From:Journal of the Korean Society of Coloproctology
2010;26(2):93-97
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Inositol hexaphosphate (IP6) is a naturally occurring polyphosphorylated carbohydrate that has been shown to suppress the growth of epithelial cancer. Because IP6 is a dietary phytochemical present in cereals, soy, legumens, and fiber-rich foods, we evaluated the efficacy of IP6 against colon cancer formation. METHODS: HT-29 cells were injected into nude mice. The animals were fed a normal diet (group 1), a low IP6 diet (group 2), and a high IP6 diet (group 3) for 4 wk. Body weight, tumor volume, tumor growth rate, growth inhibition rate, and therapeutic ratio were monitored after injection of HT-29 cells. RESULTS: HT-29-cell human-colon-carcinoma xenograft mice treated with IP6 showed a significant reduction in tumor growth rate, irrespective of the IP6 dose compared to normal diet group. Compared with the control group, group 3 showed a significant reduction (45%) in tumor volume. In the therapeutic ratio gain profiles, IP6 diet groups showed a significant alteration of therapeutic ratio when compared with the normal diet group (0% vs. 11%, P=0.014). In the body weight gain profiles, group 3 showed a significant reduction of body weight compared with the other two groups (20.25 g vs. 21.6 g, 21.7 g, P=0.009). Groups 1 and 2 showed similar changes in body weight. Tumor xenografts from IP6-fed mice showed significantly decreased cancer formation and growth, but increased toxicity was noted for high doses of IP6. CONCLUSION: These results indicate that in the future, IP6 could be an effective chemopreventive or chemotherapeutic agent for use in the treatment of colon cancer.
