Effect of Apocynin on Acute Lung Injury in Rats Given Interleukin-1alpha Intratracheally.
10.4046/trd.2011.70.6.482
- Author:
Young Man LEE
1
Author Information
1. Department of Physiology, The Catholic University of Daegu School of Medicine, Daegu, Korea. leeym@cu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
apocynin;
NADPH Oxidase;
Acute Lung Injury;
Neutrophils
- MeSH:
Acetophenones;
Acute Lung Injury;
Animals;
Bronchoalveolar Lavage;
Cytosol;
Free Radicals;
Humans;
Interleukin-1;
Interleukin-1alpha;
Lung;
Lung Injury;
Myristic Acid;
NADPH Oxidase;
Neutrophils;
Oxidative Stress;
Phorbols;
Phospholipases A2;
Rats;
Rats, Sprague-Dawley;
Superoxides;
Trachea
- From:Tuberculosis and Respiratory Diseases
2011;70(6):482-489
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Based on the assertion that apocynin diminishes acute lung injury (ALI) by inhibition of NADPH oxidase, the effect of apocynin was tested in interleukin-1alpha (IL-1)-induced ALI in rats. METHODS: IL-1 was insufflated into the trachea of Sprague-Dawley rats to induce ALI, and apocynin (8 mg/kg) was given intravenously for inhibition of NADPH oxidase. In addition, we determined whether apocynin inhibited generation of superoxide anions from isolated human neutrophils. Five hours after IL-1 instillation, lung injury parameters, expression of cytosolic phospholipase A2 (cPLA2) by cells from bronchoalveolar lavage (BAL), an index of oxidative stress in lung tissues (gamma-glutamyltranspeptidase, activity), and ultrastructure of alveolar type II (AT II) cells were evaluated. RESULTS: Apocynin decreased the generation of free radicals from phorbol myristate (PMA)-activated neutrophils in vitro, but did not ameliorate ALI. IL-1 induced enhancement of the expression of cPLA2 on neutrophils was not altered by apocynin. CONCLUSION: Apocynin induced suppression of the generation of superoxide anions from neutrophils by inhibition of NADPH oxidase does not attenuate IL-1-induced ALI in rats.
