Comparison between Two Sets of Primer for HBV-DNA by Polymerase Chain Reaction (PCR).
- Author:
Won Seon HAN
1
;
Kyong Seop SHIN
;
Bo Ra SON
Author Information
1. Department of Clinical Pathology, College of Medicine, Chungbuk National University, Cheongju, Korea.
- Publication Type:Original Article
- Keywords:
HBV-PCR;
Primer;
Mutation
- MeSH:
Alanine Transaminase;
Diagnosis;
Hepatitis, Chronic;
Humans;
Polymerase Chain Reaction*
- From:Korean Journal of Clinical Pathology
1998;18(4):614-619
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The HBV-PCR assay seems to be potentially valuable diagnostic tool for the evaluation of variable serologic status. However, the selection of the primer for HBV-PCR test may be very important because they can influence the HBV-PCR positivity. METHODS: We compared the results of primer HBV1/2 including famous 1896 and 1899 mutation sites with those of primer PHBV1/2 at precore/core region. HBV-PCR was tested in 87 HBsAg-positive patients using two sets of primers. The results were evaluated according to the primers and also compared the results with the clinical diagnosis and the alanine aminotransferase (ALT) level. RESULTS: The positive rate of PHBV primer was higher than HBV primer including mutation sites (nucleotide 1896 and 1899) in HBeAg-negative patients. According to the clinical diagnosis, the sensitivity of PHBV primer was higher than that of HBV primer in chronic hepatitis patients. There was no significant correlation between ALT level and HBV-PCR results. CONCLUSIONS: It is important that the selection of primer in HBV-PCR is important, because the primer including mutation sites may result in false negative results. PHBV primer used in this study could be useful for the detection of HBV-DNA by HBV-PCR.
