Sister Chromatid Exchanges in Lymphocytes on Normal Human Blood culture with Mercury chloride or Methylmercury Chloride.
- Author:
Dai Ha KOH
1
Author Information
1. Department of Preventive Medicine and Public Health, Chonbug National University Medical School, Korea.
- Publication Type:Original Article
- MeSH:
Animals;
Carcinogens;
Chromatids;
Chromosome Aberrations;
DNA;
Female;
Humans;
Humans*;
Lymphocytes*;
Mercuric Chloride;
Mutagens;
Ovulation;
Reproduction;
Siblings*;
Sister Chromatid Exchange*
- From:Korean Journal of Preventive Medicine
1984;17(1):245-250
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Reciprocal exchanges of DNA in sister chromatids (SCEs) are induced by various carcinogens and mutagens, although the quantitative relationship between the number of mutations and SCEs induced varies among chemicals. Nevertheless, the analysis of SCEs production by various agents often proposed as a sensitive and quantitative assay for mutagenicity and cytotoxicity. Mercury, even if which has no evidences for mutagenicity and carcinogenicity, is reported to exert women cytotoxic effects, such as chromosomal aberrations or bad influences to ovulation and reproduction in experimental animals, etc. In this study, tests for sister chromatid exchanges have been carried out on normal human lymphocytes in whole blood culture to add mercury chloride (HgCl2) or methylmercury chloride(CH3 HgCl) for 72 hr. The results indicate the dose-dependent relationship between the frequencies of SCEs and the concentrations of HgCl2, CH3HgCl and 5-bromo-2-deoxyuridine (BrdU). Lymphocyte proliferation has depressed in the higher concentration of mercury.
