Effect of Tumor Necrosis Factor-alpha Gene Transduced Bladder Tumor Cell Xenograft on Survival of C3H/He Mice.
- Author:
Seok Soo PYUN
1
;
Eun Sik LEE
;
Chongwook LEE
Author Information
1. Seoul National College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
xenograft;
tumor growth;
gene therapy;
tumor necrosis factor-alpha
- MeSH:
Animals;
DNA, Complementary;
Enzyme-Linked Immunosorbent Assay;
Genetic Therapy;
Heterografts*;
Humans;
Mice*;
Parents;
Reverse Transcription;
Tumor Necrosis Factor-alpha*;
Urinary Bladder Neoplasms*;
Urinary Bladder*;
Zidovudine
- From:Korean Journal of Urology
1996;37(12):1333-1338
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To evaluate the effect of tumor necrosis factor-alpha (TNF-alpha)gene transduced bladder tumor cells on survival of C3H/He mice. MATERIALS AND METHODS: The complete cDNA of TNF-alpha was introduced to mouse bladder tumor cell line(MBT-2) using a retroviral vector. Gene transfer and expression were confirmed by polymerase chain reaction(PCR) and reverse transcription polymerase chain reaction(RT-PCR). Produced TNF-alpha was measured by ELISA. TNF-alpha gene transduced and parental cells were xenografted in C3H/He mice. Implantation and growth rate and survival of experimental animals were analysed. RESULTS: Successful gene transfer and expression were confirmed. TNF-G gene transduced MBT-2 cells secreted TNF-alpha at the concentration of 47.4pg/106cells/24hours. The implantation and growth rate of TNF-t gene transduced MBT-2 cells were much lower than those of parental cells and survival of experimental animals was different between TNF-t gene transduced MBT-2 and parental cells(P<0.05). CONCLUSION: This study shows potential advantages of localized TNF-a secretion to induce potent antitumorigenic response. Tumor cell targeted TNF-a gene therapy could become a useful tool in treatment of bladder cancer.
