Effect of Transduced Tumor Necrosis Factor-alpha Gene Expression on Growth of Human Bladder Tumor Cell Lines.
- Author:
Haewon LEE
1
;
Eun Sik LEE
;
Chongwook LEE
Author Information
1. Seoul national University Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
cell growth;
gene therapy;
tumor necrosis factor-alpha(TNF-alpha)
- MeSH:
Cell Line*;
DNA, Complementary;
Gene Expression*;
Genetic Therapy;
Humans*;
Leukemia Virus, Murine;
Necrosis;
Neomycin;
Parents;
Reverse Transcription;
Tumor Necrosis Factor-alpha*;
Urinary Bladder Neoplasms*;
Urinary Bladder*;
Zidovudine
- From:Korean Journal of Urology
1996;37(12):1339-1344
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To evaluate the effect of transduced tumor necrosis factor-a(TNF-a) gene expression on growth of human bladder tumor cell lines in vitro. MATERIALS AND METHODS: The complete cDNA of TNF-a was introduced to three human bladder tumor cell lines(F-24, J-82, HT-1197) using a retroviral vector, a recombinant form of Molony murine leukemia virus with TNF-a and Neo gene and transfected cells were selected by exposure to neomycin analog G418. Gene transfer and expression were confirmed by polymerase chain reaction(PCR) and reverse transcription polymerase chain reaction(RT-PCR)-Southern blotting. Cell growth was measured by MTT assay Result is Successful gene transfer and expression were confirmed in all three cell bladder tumor lines. Growth of transfected cells were compared with parental cell lines and no differences were found in all three cell lines(p>0.05). CONCLUSION: Expression of transduced TNF-t gene could not show any effect on growth of human bladder tumor cells in vitro.
