Adhesion of CD40-stimulated Germinal Center B Cells to HK Cells Employs the CD11a/CD18-CD54 Interactions.
- Author:
Joonhee LEE
1
;
Jongseon CHOE
Author Information
- Publication Type:Original Article
- Keywords: Follicular dendritic cell; germinal center B cell; CD40; CD11a/CD18-CD54
- MeSH: Antibodies; Antibodies, Monoclonal; B-Lymphocytes*; Cell Communication; Cell Differentiation; Cell Proliferation; Dendritic Cells, Follicular; Germinal Center*; Intercellular Signaling Peptides and Proteins
- From:Immune Network 2003;3(3):176-181
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: The molecular basis of follicular dendritic cells (FDC)-germinal center (GC) B cell interaction is largely unknown, although this cellular interaction is thought to be important for the whole process of GC B cell differentiation. METHODS: Using FDC-like cells, HK, and highly purified GC B cells, we attempted to identify the molecules that play critical roles in the interactions between FDC and B cells. GC B cells were co-cultured with HK cells and soluble CD154 in the presence or absence of various function-blocking monoclonal antibodies to examine their effect on GC B cell binding to HK cells and B cell proliferation. RESULTS: Anti-CD11a and anti-CD54 antibodies inhibited GC B cell binding to HK cells while anti-CD49d and anti-CD106 antibodies did not. GC B cell proliferation was not impaired by the disruption of GC B cell-HK cell adherence. CONCLUSION: Our results suggest that CD11a/CD18-CD54 interactions play an important roles in the initial binding of GC B cells to FDC and diffusible growth factors from FDC may be responsible the massive proliferation of GC B cells.
