The Study of MHC class I Restricted CD8+ T Cell Mediated Immune Responses against Mycobacterium tuberculosis Infection: Evidence of M. tuberculosis Specific CD8+ T Cells in TB Patients and PPD+ Healthy Individuals.
- Author:
Jang Eun CHO
1
;
Kyung Wha LEE
;
Seung Kyu PARK
;
Seon Hee CHEON
;
Sang Nae CHO
;
Sungae CHO
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: Mycobacterium tuberculosis; CD8+ T cells; peptide epitopes; HLA-A*0201; IFN-gamma elispot assay
- MeSH: Cell Line; Enzyme-Linked Immunospot Assay; Epitopes; HLA-A2 Antigen; Humans; Mycobacterium tuberculosis*; Mycobacterium*; Peptides; T-Lymphocytes*; Tissue Donors; Tuberculosis*
- From:Immune Network 2003;3(3):235-241
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: The protective immunity against tuberculosis (TB) involves both CD4+ T cells and CD8+ T cells. In our previous study, we defined four Mycobacterium tuberculosis derived peptide epitopes specific for HLA-A*0201 restricted CD8+ T cells (ThyA30-38, RpoB127-135, 85B15-23, PstA175-83). In this study, we investigated the immune responses induced by these peptide specific CD8+ T cells in latently and chronically infected people with TB. METHODS: We characterized these peptide specific CD8+ T cell population present in PBMC of both TB patients and PPD healthy people using IFN-gammaelispot assay, intracellular staining and HLA-A2 dimer staining. RESULTS: The frequency of peptide specific CD8+ T cell was in the range of 1 to 25 in 1.7x10(5) PBMC based on ex vivo IFN-gamma elispot assay, demonstrating that these peptide specific CD8+ T cell responses are induced in both TB patients and PPD people. Short term cell lines (STCL) specific for these peptides proliferated in vitro and secreted IFN-gamma upon antigenic stimulation in PPD+ donors. Lastly, HLA-A*0201 dimer assays indicated that PstA175-83 specific CD8+ T cell population in PPD+ healthy donors is heterogeneous since approximately 25~33% of PstA175-83 specific CD8+ T cell population in PPD+ healthy donors produced IFN-gamma upon peptide stimulation. CONCLUSION: Our results suggest that MHC class I restricted CD8+ T cell mediated immune responses to M. tuberculosis infection are induced in both TB patients and PPD+ people; however, the CD8+ T cell population is functionally heterogeneous.
