Chimerism Proven by Flow Cytomety in the Setting of an ABO Discrepancy: A Case Report.
- Author:
Eun Sun JEONG
1
;
Chang Jin MOON
;
Duck CHO
;
Jong Tae PARK
;
Dong Wook RYANG
Author Information
1. Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea. dcho@chonnam.ac.kr
- Publication Type:Case Report
- Keywords:
Chimera;
ABO discrepancy;
Flow cytometry
- MeSH:
Animals;
Chimera;
Chimerism;
DNA;
Exons;
Female;
Flow Cytometry;
Genotype;
Histocompatibility Testing;
Humans;
Leukocytes;
Mice;
Microsatellite Repeats;
Phenotype;
Pregnant Women
- From:Korean Journal of Blood Transfusion
2009;20(3):247-252
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Chimerism is an important, yet uncommon cause of ABO phenotype/genotype discrepancies. The propositus was a 28 year-old pregnant women who had an ABO discrepancy, expressing an A(weak)B RBC phenotype and AB reverse type. Sequencing of ABO exons 6 and 7 revealed a B101/O01 genotype. Neither analysis of 9 short tandem repeats (STR) loci nor HLA typing on DNA extracted from white blood cells demonstrated evidence of chimerism. However, flow cytometric analysis using a PE-conjugated anti-A mouse monoclonal antibody detected a small population (3.8%) of red cells expressing normal A antigen. Based on this, we suggest that flow cytometric analysis is an effective method for the identification of small chimeric populations of RBCs in the immunohematology laboratory.
