The Effect of Platelet Rich Plasma from Bone Marrow Aspirate with Added Bone Morphogenetic Protein-2 on the Achilles Tendon-Bone Junction in Rabbits.
10.4055/cios.2011.3.4.325
- Author:
Hak Jun KIM
1
;
Hyok Woo NAM
;
Chang Yong HUR
;
Misu PARK
;
Hee Seok YANG
;
Byung Soo KIM
;
Jung Ho PARK
Author Information
1. Department of Orthopaedic Surgery, Korea University College of Medicine, Seoul, Korea. canall@korea.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Bone-tendon junction;
Achilles tendon;
Bone marrow derived platelet rich plasma;
Bone morphogenetic protein
- MeSH:
Achilles Tendon/*injuries;
Animals;
Bone Marrow;
Bone Morphogenetic Protein 2/*therapeutic use;
Calcaneus/*injuries;
Male;
*Platelet-Rich Plasma;
Rabbits
- From:Clinics in Orthopedic Surgery
2011;3(4):325-331
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: To determine if exogenously injected bone marrow derived platelet-rich plasma (PRP) plus bone morphogenetic protein (BMP)-2 could accelerate the healing of bone-tendon junction injuries and increase the junction holding strength during the early regeneration period. METHODS: A direct injury model of the bone-tendon junction was made using an Achilles tendon-calcaneus bone junction in a rabbit. In the PRP/BMP-2/fibrin group, 0.05 mL of bone marrow derived PRP and 100 ng/mL of BMP-2 both incorporated into 0.1 mL of fibrin glue were injected into Achilles tendon-calcaneus bone junctions. The effect of the intervention was tested by comparing the results of an intervention group to a control group. The results of biomechanical testing, and histological and gross analyses were compared between the 2 groups at the following time points after surgery: 2 weeks, 4 weeks, and 8 weeks. RESULTS: Histologic examinations showed that woven bone developed in tendon-bone junctions at 2 weeks after surgery in the PRP/BMP-2/fibrin group. Mechanical test results showed no significant difference between the PRP/BMP-2/fibrin and control groups at 2 and 4 weeks after surgery, but the mean maximal load in the PRP/BMP-2/fibrin group was significantly higher than in the control group (p < 0.05) at 8 weeks after surgery. CONCLUSIONS: Bone marrow derived PRP and BMP-2 in fibrin glue accelerated healing in a rabbit model of tendon-bone junction injury.
