Sputum metabolomics study in patients with occupational coal workers′ pneumoconiosis
10.20001/j.issn.2095-2619.20250601
- VernacularTitle:职业性煤工尘肺患者痰液代谢组学研究
- Author:
Yiming ZHANG
1
;
Qiufang QU
;
Qingnan ZHOU
;
Shuhan GUO
;
Le LIU
;
Yuke WANG
;
Zhenlin HE
;
Sanqiao YAO
Author Information
1. Xinxiang Medical University, Xinxiang, Henan 453003, China
- Publication Type:Journal Article
- Keywords:
Coal workers' pneumoconiosis;
Sputum;
Metabolomics;
Differential metabolites;
Metabolic pathways;
Biomarkers
- From:
China Occupational Medicine
2025;52(3):241-248
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the sputum metabolic profiles of patients with occupational coal workers' pneumoconiosis (CWP) by an untargeted metabolomics method, and to identify relevant differential metabolic pathways and potential biomarkers. Methods A total of 12 male patients with stage Ⅰ CWP were selected as the CWP group, and 16 healthy male individuals were selected as the control group, using a judgmental sampling method. Sputum metabolites of individuals in both groups were detected to perform non-targeted metabolomic analysis using the ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Differential metabolites (DMs) and their pathways were screened using principal component analysis, partial least squares discriminant analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Potential biomarkers were analyzed and identified via the receiver operating characteristic curve (ROC). Results There were apparent metabolic alterations observed in sputum of CWP patients compared with healthy controls. In the positive ion mode, a total of 42 DMs were identified in sputum from CWP patients, including 19 downregulated and 23 upregulated metabolites. In the negative ion mode, a total of 25 DMs were identified in sputum from CWP patients, including 16 downregulated and 9 upregulated metabolites. KEGG enrichment analysis of sputum from CWP patients showed that seven DMs pathways were enriched in ABC transporters, histidine metabolism, phenylalanine metabolism, arachidonic acid metabolism, linoleic acid metabolism, purine metabolism, and oxidative phosphorylation, involving 26 DMs. ROC analysis indicated that 16(R)-hydroxyarachidonic acid, pyrophosphate, and 2-hydroxyphenylacetate of these 26 DMs may serve as potential biomarkers for CWP. Conclusion Sputum metabolomic profiles were altered in CWP patients compared with healthy controls. The potential biomarkers of CWP prevention and treatment are 16(R)-hydroxyarachidonic acid, pyrophosphate, and 2-hydroxyphenylacetate.
