Establishment, applicability analysis and verification of DNA barcoding for cell species identification
- VernacularTitle:细胞种属鉴别DNA条形码法的建立、适用性分析以及验证
- Author:
WU Xueling
- Publication Type:Journal Article
- Keywords:
Cell species;
Identification;
DNA barcode;
Mitochondria
- From:
Chinese Journal of Biologicals
2025;38(09):1056-1062+1071
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a DNA barcode method for cell species identification, analyze and verify the applicability of the method, so as to use it for cell species identification in biological products production.Methods The mitochondrial cytochrome C oxidase subunit 1(COI) gene was selected as the target gene, and two sets of primers(V primer and L primer)were chosen for specific amplification of this target gene. The amplification products were sequenced, and their sequences were aligned with the database to determine the species of cells being detected.Results The two sets of primers exhibited differential amplification abilities across various cell species. V primer and L primer successfully detected cells from mice,hamsters, guinea pigs, gophers/prairie dogs, cats, cows, chickens, and ducks. Additionally, V primer could also be utilized for detecting cells from monkeys, rats, dogs, mink, pigs, rabbits and humans. L primer could also be utilized for the detection of insect-derived cells. This method presented a detection sensitivity of 10 cells and could detect cross contamination at a hostto-contaminated cell ratio of 10∶1. Review verification demonstrated that four units successfully identified the species of eight unknown cells using this DNA barcode method.Conclusion The established DNA barcode method is effective for identifying cell species from 17 different types, providing a new technical approach to ensure the accurate use of cells in biological products production.
