Construction of an iron-based metal organic framework material and investigation of adjuvant effects
10.13200/j.cnki.cjb.004232
- VernacularTitle:基于铁基金属有机框架材料的构建及其佐剂效应
- Author:
ZHANG Ji
- Publication Type:Journal Article
- Keywords:
Iron-based metal organic framework(MOF) material;
Adjuvant;
Humoral immunity;
Cellular immunity
- From:
Chinese Journal of Biologicals
2025;38(09):1035-1042
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize an iron-based metal organic framework(MOF) material and investigate its adjuvant effect on immune response in mice. Methods The iron-based MOF material was synthesized via the hydrothermal reaction method, of which the morphology and composition were characterized by transmission electron microscope(TEM), scanning electron microscope(SEM), dynamic light scattering particle analyzer(DLS), X-ray photoelectron spectrometer(XPS), and specific surface area analysis(Brunauer Emmett Teller, BET). Using ovalbumin(OVA) as immunogen, 20 female BALB/c mice were randomly divided into iron-based MOF + OVA group(60 μg iron-based MOF + 20 μg OVA), Alu + OVA group(50 μg Alu+ 20 μg OVA), OVA group(20 μg OVA), and blank group(0. 9% normal saline), and were injected intramuscularly through the lateral thigh at 0, 14 and 28 d, respectively, with 100 μL/mouse. Blood samples were collected from the tail vein at 7, 21 and42 d after the initial immunization, the serum was separated, and the specific IgG antibody level was determined by ELISA.At 42 d after the initial immunization, the levels of specific IgG subtype antibodies in serum, IFNγ secreted by splenocytes,as well as IFNγ, IL-2 and IL-4 secreted by CD4~+T and CD8~+T cells were detected by ELISA, ELISPOT and flow cytometry,respectively. The body mass of mice at 0, 7, 14, 21, 28, 35, and 42 d after the initial immunization was measured, and the behavior of mice in various groups was observed. Results The synthesized iron-based MOF material exhibited a spherical morphology, an average particle size of approximately 129. 4 nm, a specific surface area of 16. 7 m~2/g and a pore diameter of7. 73 nm, containing C, N, S and Fe elements with more content of Fe element. At 42 d after the initial immunization, there was no significant difference in IgG level between iron-based MOF + OVA group and Alu + OVA group(F = 1. 00, P > 0. 05),but IgG2a and IgG2b levels increased significantly in iron-based MOF + OVA group(F = 16. 90 and 16. 57, respectively,each P < 0. 05);the level of IFNγ secreted by spleen cells increased significantly(F = 19. 47, each P < 0. 05);the level of IFNγ secreted by CD4~+T cells increased significantly(F = 14. 94, P < 0. 01), but that secreted by CD8~+T cells had no significant difference(F = 1. 00, P > 0. 05), and the levels of IL-2 and IL-4 secreted by CD8~+T cells increased significantly(F =100. 00 and 945. 80, each P < 0. 001). During the whole immunization process, there were no abnormal behaviors such as depilation or refusal to eat, and the body mass of mice in various groups changed little. Conclusion The prepared iron-based MOF material is simple to synthesize, stable in structure with nano-scale size, which can elicit humoral and cellular immune responses in mice, and is expected to become a new vaccine adjuvant.
