The role of human umbilical cord-derived mesenchymal stem cells transplantation in alleviating radiation-induced ovarian injury
10.13491/j.issn.1004-714X.2025.04.019
- VernacularTitle:人脐带间充质干细胞对辐射致卵巢损伤的修复作用
- Author:
Mei ZHANG
1
;
Chao YANG
1
;
Bo CHENG
1
;
Jianan WANG
1
;
Yinghao MA
1
;
Zheng ZHANG
1
;
Qingxiang HOU
1
;
Li MA
1
Author Information
1. The PLA Rocket Force Characteristic Medical Center, Beijing 100088, China.
- Publication Type:OriginalArticles
- Keywords:
Radiation;
Ovarian injury;
Umbilical cord-derived mesenchymal stem cell;
Repair
- From:
Chinese Journal of Radiological Health
2025;34(4):584-589
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using female mice to investigate the reparative effects of human umbilical cord mesenchymal stem cells on radiation-induced ovarian injury. Methods Mice were randomly divided into three groups: a blank control group, a radiation model group, and a cell therapy group. Mice in the radiation model group and the cell therapy group received a single whole-body irradiation of 5 Gy X-rays. Within 2 hours post-irradiation, mice in the cell therapy group underwent ovarian transplantation of UC-MSCs. On days 1, 7, and 14 post-irradiation, body weight was measured, ovarian index was calculated, histopathological changes in ovarian tissue were examined, serum levels of reproductive hormones (follicle-stimulating hormone, anti-Müllerian hormone, and estradiol) were determined, and the colonization of implanted UC-MSCs in the mice was observed. Results On days 1, 7, and 14 post-irradiation, both the cell therapy group and the radiation model group showed decreased body weight compared to the blank control group (P < 0.05). On day 1 post-irradiation compared to day 1 pre-irradiation within the same group, the radiation model group exhibited a greater decrease in body weight than the cell therapy group (P < 0.05). On days 1, 7, and 14 post-irradiation, the ovarian index decreased in both the radiation model group and the cell therapy group compared to the blank control group (P < 0.05). On days 7 and 14 post-irradiation, the ovarian index in the cell therapy group was significantly higher than that in the radiation model group (P < 0.05). Ovarian tissue in the radiation model group exhibited atrophy and a reduction in the number of follicles at all stages. In contrast, follicles in the cell therapy group were large and abundant. On days 1, 7, and 14 post-irradiation, serum follicle-stimulating hormone levels in the cell therapy group were lower than those in the radiation model group, while anti-Müllerian hormone and estradiol levels were higher than those in the radiation model group (P < 0.01). In vivo fluorescence imaging demonstrated that UC-MSCs successfully colonized the ovarian tissue on days 1, 7, and 14 after transplantation. Conclusion UC-MSCs exert a repair effect on radiation-induced ovarian injury in mice.
