Uncertainty evaluation for the determination of melatonin in dietary supplements by ultra high performance liquid chromatography tandem mass spectrometry
10.19428/j.cnki.sjpm.2025.25071
- VernacularTitle:超高效液相色谱质谱联用法测定保健食品中褪黑素的不确定度评定
- Author:
Shaoming JIN
1
;
Xiao NING
1
Author Information
1. Key Laboratory of Food Quality and Safety, State Administration for Market Regulation, Institute for Food and Cosmetics Testing, National Institutes for Food and Drug Control, Beijing 100050, China
- Publication Type:Journal Article
- Keywords:
ultra-high performance liquid chromatography tandem mass spectrometry;
melatonin;
content determination;
uncertainty;
dietary supplement
- From:
Shanghai Journal of Preventive Medicine
2025;37(7):625-628
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the sources of uncertainty in determination of melatonin in dietary supplements using ultra-high performance liquid chromatography-mass spectrometry (LC-MS) technology, to explore the effects of each component, and to improve the accuracy of the determination method. MethodsThe sources of uncertainty in establishment of a method for determining melatonin in dietary supplements using liquid chromatography-mass spectrometry were analyzed. These sources mainly included non-uniformity, balance weighing, repeatability of sample testing, solution preparation, standard curve fitting, and instrument tolerance error, etc, and the synthesis of these uncertainties was also discussed. ResultsThe factors that contributed significantly to uncertainty were mainly the process of sample preparation and curve fitting. The expanded uncertainty for 500 mg melatonin tablets in content determination was 15.624 ng·mL-1 (P=95%, k=2). ConclusionThe uncertainty of measuring melatonin content by liquid chromatography-mass spectrometry is mainly introduced in the context of standard curve fitting, solution preparation, and sample pretreatment. The experimental process should be strictly standardized, steps should be simplified, and the accuracy of experimental measurement results should be improved.
