Effects of superoxide dismutase inhibition of AFP expression on the malignant biological behavior of PLC/PRF/5 liver cancer cells
- VernacularTitle:超氧化物歧化酶抑制AFP表达对肝癌细胞PLC/PRF/5恶性生物学行为的影响
- Author:
Yi CHEN
1
,
2
,
3
;
Baoying CHEN
2
;
Yuli ZHOU
2
,
3
;
Haixia XU
4
;
Yu CAO
2
;
Yue GU
2
;
Mingyue ZHU
1
,
2
,
3
;
Mengsen LI
1
,
2
Author Information
1. Key Laboratory of Tropical Translational Medicine of Ministry of Education，Haikou 571199，China
2. School of Basic Medicine and Life Sciences，Hainan Medical University，Haikou 571199，China
3. Hainan Provincial Key Laboratory of Carcinogenesis and Intervention，Haikou 571199，China
4. Dept. of Spinal Surgery，the First Affiliated Hospital of Hainan Medical University，Haikou 570102，China
- Publication Type:Journal Article
- Keywords:
superoxide dismutase;
alpha fetoprotein;
hepatocellular carcinoma;
malignant behavior;
anti-cancer drugs
- From:
China Pharmacy
2025;36(17):2120-2126
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effect of superoxide dismutase （SOD） administration on the malignant behavior of PLC/PRF/5 liver cancer cells， and analyze the correlation between SOD and alpha-fetoprotein （AFP） expression， to provide new ideas for targeting AFP with SOD as a drug for hepatocellular carcinoma. METHODS Normal human liver cells L-02， AFP- negative human liver cancer cells HLE， and AFP-positive human liver cancer cells PLC/PRF/5 were used as experimental cells. Western blot assay and SOD activity detection kit were used to detect the expression of AFP， SOD and activity of SOD in cells before and after changing AFP expression； the effects of different concentrations of SOD [0 （control）， 0.188， 0.375， 0.75， 1.5， 3 U/mL] administration on the migration and proliferation of PLC/PRF/5 cells were detected using cell scratch assay and CCK-8 assay. The effects of SOD overexpression on the expression of malignant biological behavior-related proteins AFP and sarcoma virus protein （Src） in PLC/PRF/5 cells were detected using Western blot. RESULTS Compared with L-02 group and HLE group， the expression levels of SOD1 and SOD2， and SOD activity in PLC/PRF/5 cells were significantly reduced （P＜0.05）. After down-regulating AFP expression in PLC/PRF/ 5 cells， compared with PLC/PRF/5 group， the expression levels of SOD1 and SOD2， as well as SOD activity， were significantly increased in the PLC/PRF/5-shAFP group （low-expression） （P＜0.05）. After 48 hours of SOD treatment， compared with control group， the scratch healing rates of PLC/PRF/5 cells in the 0.375， 0.75， 1.5 and 3 U/mL SOD groups were significantly reduced （P＜0.05）； after 72 hours of SOD treatment， compared with control group， the scratch healing rates of PLC/PRF/5 cells in the 0.375， 0.75， and 1.5 U/mL SOD groups were significantly reduced （P＜0.05 or P＜0.01）. Compared with control group， proliferation rates of PLC/PRF/5 cells were significantly reduced in the 0.375， 0.75， 1.5 and 3 U/mL SOD groups （P＜0.05 or P＜0.01）. Compared with the PLC/PRF/5 group before up-regulating SOD1 and SOD2 expression， the expression levels of AFP and Src in the PLC/PRF/5-oeSOD1 and PLC/PRF/5-oeSOD2 groups （over-expression） after up-regulating SOD1 and SOD2 expression were significantly reduced （P＜0.05）. CONCLUSIONS A certain concentration of SOD can inhibit malignant behavior such as migration and proliferation of PLC/PRF/5 cells， and the expression level and activity of SOD are negatively correlated with AFP.
