Preparation and evaluation of long-acting light-protective nanogel based on fullerenol-cerium oxide composite system
- VernacularTitle:基于富勒醇-氧化铈复合体系的长效光防护纳米凝胶的制备与评价
- Author:
Tianlong ZHANG
1
;
Jia LIU
1
;
Qing ZHAO
1
;
Yue ZHOU
2
;
Ming YANG
2
;
Qianyu LUO
3
Author Information
1. College of Pharmacy，North Sichuan Medical College，Sichuan Nanchong 637000，China
2. Dept. of Pharmacy，the Affiliated Hospital of North Sichuan Medical College，Sichuan Nanchong 637000，China
3. Dept. of Pharmacy，Jintang First People’s Hospital，Chengdu 610000，China
- Publication Type:Journal Article
- Keywords:
nanogel;
fullerenol;
cerium oxide;
high altitude ultraviolet damage;
antioxidant;
light protection
- From:
China Pharmacy
2025;36(17):2106-2112
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To develop a long-acting light-protective nanogel with both physical barrier and chemical clearance functions， and evaluate its performance. METHODS The photoprotective nanogel composed of mussel mucin and sodium hyaluronate was constructed based on a fullerenol-cerium oxide composite nano system， namely fullerenol-cerium oxide nanogel （FCN）， and was characterized. The antioxidant capacity of FCN was evaluated using in vitro free radical scavenging experiments； its UV shielding ability was assessed by using an SPF value detector； its biosafety was assessed according to the requirements of the Guidelines for Drug Safety Evaluation； skin adhesion was assessed using small animal 3D live imaging technology； its sun protection ability was assessed through skin sunscreen detection and histopathological observation. RESULTS The average particle sizes of cerium oxide and fullerenol nanoparticles in FCN were about 20 and 10 nm， respectively， and FCN exhibited good UV absorption and free radical scavenging abilities. SPF value of FCN was 58.95±0.82， and the ultraviolet A protection level value was 6.21±0.15. No pathogenic colonies such as Staphylococcus aureus， were detected in the nanogel， and the contents of lead， arsenic， mercury and cadmium all met the standards for pharmaceutical excipients； FCN group did not show any irritating reactions such as erythema， edema， or desquamation； blood biochemical indicators of the FCN group were within the normal reference range. The material clearance rate of mice in the artificial sweat flushing group was less than 30%， while the material clearance rate of mice in the dry cleaning group reached about 92%. The mice in the protective group did not show obvious erythema or ulcer formation throughout the experiment. Histopathology showed that the fibers were arranged in an orderly manner， and the number of collagen fibers was close to that of the control group. CONCLUSIONS The FCN formulation constructed in this study meets the relevant requirements of the Chinese Pharmacopoeia， has good safety and skin compatibility， and achieves dual synergistic protection of UV shielding and free radical scavenging.
