Exploring effective components and therapeutic mechanism of Chaihu-Guizhi-Ganjiang decoction in treatment of chronic non-atrophic gastritis by UHPLC-Q-TOF/MS combined with network pharmacology
10.12206/j.issn.2097-2024.202312061
- VernacularTitle:基于UHPLC-Q-TOF/MS结合网络药理学研究柴胡桂枝干姜汤治疗慢性非萎缩性胃炎的物质基础及疗效机制
- Author:
Tao WEN
1
;
Guangyang JIAO
2
;
Mengpei ZHAO
1
;
Xiaoqiang YUE
3
;
Feng ZHANG
1
;
Wansheng CHEN
2
,
4
Author Information
1. Department of Pharmacy, Medical Support Center.
2. Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
3. Department of Pharmacy, Medical Security Center, 1b. Department of Traditional Chinese Medicine, The Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China.
4. Department of Pharmacy, Medical Support Center
- Publication Type:Originalarticles
- Keywords:
Chaihu-Guizhi-Ganjiang decoction;
chronic non-atrophic gastritis;
UHPLC-Q-TOF/MS;
network pharmacology
- From:
Journal of Pharmaceutical Practice and Service
2025;43(9):455-462
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effective components and therapeutic mechanism of Chaihu-Guizhi-Ganjiang decoction in treating chronic non-atrophic gastritis. Methods The primary and secondary ion fragments of chemical components of Chaihu-Guizhi-Ganjiang decoction were obtained by UHPLC-Q-TOF/MS. Comparing with reference standards and literature information, a comprehensive characterization of the chemical constituents of Chaihu-Guizhi-Ganjiang decoction was conducted. Then, the network pharmacology approach was applied to explore the therapeutic mechanism of Chaihu-Guizhi-Ganjiang decoction in treatment of chronic non-atrophic gastritis based on the components in plasma and verified by immunohistochemical results. Results A total of 24 absorbed components of Chaihu-Guizhi-Ganjiang decoction were characterized, including 11 flavonoid glycosides, 3 fatty acids, 3organic acids, 2 gingerols, 2 flavonoids and, 1 each of fatty aldehydes, triterpenoids and amino acids, which mainly acted on TNF-α, IL-6, STAT3, and PTGS2. It exerted therapeutic effects by modulating signaling pathways, including the IL-17 signaling pathway and the AGE-RAGE signaling pathway, etc. Conclusion This study provided the first exploration of the effective components and therapeutic mechanism of Chaihu-Guizhi-Ganjiang decoction in treatment of chronic non-atrophic gastritis by UHPLC-Q-TOF/MS, which could offer scientific references for its further research.
