Separation of chemical constituents of Tibetan medicine Fallopia aubertii L.Henry Holub by two-dimensional liquid chromatography
10.12206/j.issn.2097-2024.202412065
- VernacularTitle:二维液相色谱法分离藏药木藤蓼的化学成分
- Author:
Shenghui SHI
1
;
Xiao LIU
1
;
Dong CHEN
1
;
Dijun JI
2
,
3
;
Qian MA
2
,
3
;
Yongchang LU
2
,
3
Author Information
1. Department of Pharmacy, Qinghai Provincial People’s Hospital, Xining 810007, China.
2. School of Pharmacy, Qinghai Nationalities University, Xining 810007, China
3. Key Laboratory of Plant Resources of Qinghai-Tibet Plateau in Chemical Research, Xining 810007, China.
- Publication Type:Originalarticles
- Keywords:
Fallopia aubertii L.Henry Holub;
two-dimensional liquid chromatography（2D-LC）;
chemical constituents;
xanthine oxidase
- From:
Journal of Pharmaceutical Practice and Service
2025;43(9):443-448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the chemical constituents of Fallopia aubertii L.Henry Holub. Methods The chemical constituents of Fallopia aubertii L.Henry Holub. were separated and purified by online two-dimensional preparative liquid chromatography and identified by physical and chemical constants and spectral analysis. The inhibitory activities on xanthine oxidase were determined by ultraviolet spectrophotometry. Results Ten compounds were isolated from the extract of Fallopia aubertii L.Henry Holub, including isotachioside（1）, 3,4,5-trimethoxyphenyl-（6'-O-galloyl）-O-β-D-Glucopyranoside（2）, 1-hydroxy-,4,5-1-O-[6'-O-（4''-carboxy-1'',3'',5'trihydrotrimethoxyphenylxy）-phenyl]-β-D-glucopyranoside（3）, myricetrin（4）, myricetin（5）, rutin（6）, quercetin-3-O-β-D-galactoside（7）, quercetin-3-O-β-D-glucopyranoside（8）, lyciumideA（9）, and N-trans-Feruloyltyramine（10）. The inhibitory activity test results showed that the IC50 of compound 5 was 15.92 μmol/L, and the IC50 of compound 6 was 87.36 μmol/L. Conclusion Compounds 1,2,3,4 and 8 were isolated from Medicago polymorpha for the first time. Compounds 5 and 6 had xanthine oxidase inhibitory activity.
