RhD-negative blood donors: genetic polymorphisms and testing strategies
10.13303/j.cjbt.issn.1004-549x.2025.07.011
- VernacularTitle:RhD阴性献血者的RHD基因多态性及检测策略
- Author:
Kecheng WANG
1
;
Xiaoqin WANG
1
;
Yingzhou DING
1
;
Tingting ZHANG
2
;
Ming LIU
2
;
Cheng XU
1
Author Information
1. Nantong Blood Center, Nantong 226014, China
2. Weihe Biotechnology Inc, Taizhou 225300, China
- Publication Type:Journal Article
- Keywords:
Rh blood group;
phenotype;
genotype;
RhD negative;
DEL;
RhD variants;
genetic basis
- From:
Chinese Journal of Blood Transfusion
2025;38(7):934-940
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the genetic basis of RhD-negative phenotype in the blood donor population of Nantong City. Methods: RHD genotyping was performed on 386 randomly selected RhD-negative donor samples (from a total of 676 RhD-negative donors identified between January 20, 2023, and June 28, 2024) using polymerase chain reaction (PCR), and the inconclusive results were confirmed by nucleotide sequencing. Results: Ten RHD allele types were identified: The complete deletion variant RHD
01N.01 was predominant (64.25%, 248/386); followed by RHD
01EL.01 (19.69%, 76/386). RHD
01N.03, RHD
01N.04, RHD
01N.16 and RHD
01EL.32 were frequently observed., RHD
01EL.02, RHD
01EL.08, RHD
01EL.37 and RHD
01N.25 were rare, and two exon deletion variants remained uncharacterized. The phenotypic distribution of RhD-negative blood donors was ccee (55.44%)>Ccee(31.09%)>ccEe(5.96%)>CCee(5.44%)>CcEe(1.81%)>CcEE(0.26%), and the antigen distribution trend was e(99.74%)>c(94.56%)>C(38.60%)>E(8.03%). A correlation was observed between RHD genotypes and RhCE phenotypes. Conclusion: The Nantong blood donor population exhibits unique RHD gene polymorphisms. Integrating RhCE serological phenotyping with RHD genotyping is essential for ensuring transfusion safety.
