Data analysis of HBV DNA detection proficiency testing in blood station laboratories
10.13303/j.cjbt.issn.1004-549x.2025.08.014
- VernacularTitle:血站实验室HBV DNA检测能力验证数据分析
- Author:
Yanbin WANG
1
;
Lianjun HAO
1
;
Huixian ZHANG
1
;
Ye SUN
1
;
Congya LI
1
;
Kun TANG
1
;
Xi TANG
1
Author Information
1. Hebei Blood Center, Shijiazhuang 050071, China
- Publication Type:Journal Article
- Keywords:
nucleic acid test;
system comparison;
capability verification;
PCR;
HBV DNA
- From:
Chinese Journal of Blood Transfusion
2025;38(8):1089-1093
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To design HBV DNA proficiency testing and system comparison samples with different concentration gradients, analyze their detection results in PCR detection systems, evaluate the nucleic acid detection capabilities of laboratories and differences between detection systems, and put forward suggestions for continuous quality improvement to participating laboratories. Methods: Three groups of randomly numbered proficiency testing samples (with HBV DNA reference concentrations of <2, 7.5, and 30 IU/mL respectively) were taken as the detection objects. Using nucleic acid test data from 11 provincial blood station laboratories as the source, the samples were grouped by detection system and laboratory successively, and statistical analysis was conducted. Results: Statistical analysis of the detection data of the three groups of samples based on detection systems and laboratories showed that from low to high concentration, the coincidence rate between the detection results of different detection systems and laboratories and the expected results showed an increasing trend: 38.89%, 85.90%, and 100.00%; the same system exhibited certain differences in performance among different laboratories. Conclusion: Through this proficiency testing and system comparison, it is found that there are certain differences in the detection capabilities of different laboratories and different nucleic acid test systems. Blood station laboratories should standardize processes, strengthen quality management and data analysis on the basis of being familiar with the detection performance of their detection systems, and at the same time strengthen the control of laboratory interference factors to continuously improve the nucleic acid detection capabilities of blood station laboratories.
