Establishment and application of a red blood cell gene database in regular blood donors
10.13303/j.cjbt.issn.1004-549x.2025.08.009
- VernacularTitle:固定献血者红细胞基因数据库的建立和应用
- Author:
Zhihui FENG
1
;
Xiaoyun CHI
1
;
Bin HU
1
;
Li LIU
1
;
Dawei LI
1
;
Shutao PANG
1
Author Information
1. Qingdao Blood Center, Qingdao 266071, China
- Publication Type:Journal Article
- Keywords:
red blood cell antigen genotyping;
database;
precise blood transfusion;
red blood cell transfusion refractoriness
- From:
Chinese Journal of Blood Transfusion
2025;38(8):1056-1062
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a "regular blood donor red blood cell gene database"(hereafter referred to as the "database") by applying molecular biology techniques for red blood cell antigens genotyping and utilizing information technology software, and to determine the significance and application value of this "database" in precise red blood cell transfusion. Methods: Fifteen antigens [C, c, E, e, M, N, S, s, Fy (a), Fy (b), Jk (a), Jk (b), Le (a), Le (b), P1] across six blood group systems (RHCE, MNS, FY, JK, Lewis and P1PK) were detected among 9 426 regular blood donors using the TaqMan-MGB method combined with an improved U-shaped microplate approach. With the assistance of information technology software, the "database" was integrated into the overall inventory management system of the blood supply chain. This enabled comprehensive management of regular blood donor and patient information, test results, specific antigen screening for regular blood donors, graded antigen matching between donors and patients, and rare blood type donor records. Results: The TaqMan-MGB method successfully detected paired antigens (C/c, E/e, M/N, S/s, Fy
/Fy
, Jk
/Jk
) within a single reaction well using a standardized PCR amplification protocol. This method provided a reliable testing solution for clinical institutions and empowered blood collection and supply organizations with high-throughput screening capabilities. In the blood supply chain, genotyped red blood cells accounted for 13.2% (721/5 462 U) of the total inventory, with 95.34% (348/365) originating from donors who donated two units of blood. Moreover, the “database” fulfilled 94.06% (443/471 U) of compatible transfusion requirements from medical institutions and effectively managed rare blood type donors. Conclusion: The establishment of the "database" facilitated the transition of blood compatibility testing from traditional serological methods to molecular biology-based gold standard techniques, significantly advancing the implementation of precise transfusion strategies based on multi-antigen matching between donors and patients.
