Distribution of GP (B-A-B) hybrid glycophorins in Guangdong & Guizhou minority populations

Ling WEI; Peng WANG; Jizhi WEN; Shuangshuang JIA; Yanli JI

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Distribution of GP (B-A-B) hybrid glycophorins in Guangdong & Guizhou minority populations

VernacularTitle:MNS杂交糖蛋白GP（B-A-B）在广东与贵州少数民族中的分布
Author: Ling WEI ^{1
,

2
,

3} ; Peng WANG ⁴ ; Jizhi WEN ^{1
,

2
,

3} ; Shuangshuang JIA ^{1
,

2
,

3} ; Yanli JI ^{1
,

2
,

3}
Author Information

1. Guangzhou Blood Center, Guangzhou 510095, China
2. Institute of Blood Transfusion and Hematology, Guangzhou Medical University, Guangzhou 510095, China
3. The Key Medical Laboratory of Guangzhou, Guangzhou 510095, China
4. Bijie Blood Center, Bijie 551700, China
Publication Type:Journal Article
Keywords: MNS hybrid glycophorin; GP (B-A-B); GP. Mur; high resolution melting (HRM)
From: Chinese Journal of Blood Transfusion 2025;38(8):1050-1055
CountryChina
Language:Chinese
Abstract: Objective: To investigate the distribution of GP (B-A-B) hybrid glycophorins in several Chinese minority populations from southern regions of China (Guangdong & Guizhou). Methods: Whole blood samples were collected from 536 blood donors representing 15 different Chinese ethnic minority groups, including She, Bouyei, Yi and Miao, as well as Chuanqing populations. Genomic DNA was extracted and GYP (B-A-B) genotyping was conducted by high resolution melting (HRM) minority method using the GYPB pseudoexon 3-specific primers. Direct sequencing of GYPB pseudoexon 3 was performed in the samples with variant curves. Results: Only one genotype of GP (B-A-B) hybrid glycophorins (GYP Mur/GYPB) was identified among these 536 samples. In total, 15 She (15/162, 9.26%), 18 Bouyei (18/113, 15.93%), 3 Yi (3/79, 3.80%), 3 Chuanqing (3/45, 6.67%), 2 Bai (2/42, 4.76%), 3 Miao (3/40, 7.50%), 1 Shui (1/12, 8.33%), 2 Gelao (2/12, 16.67%), 1 Tujia (1/8, 12.50%) and 1 Dong (1/6, 16.67%) blood donors with heterozygous GYP Mur allele were identified. Among 8 Hui, 5 Manchu, 2 Mongolian, 1 Yao and 1 Li donors, no GYP (B-A-B) hybrid gene carrier was found. In addition, four nucleotide polymorphisms (SNPs) were identified in 6 samples with a variant melting curve detected by HRM. Conclusion: GP. Mur is the most common type of GP (B-A-B) hybrid glycophorins among Chinese minority populations, with frequency varying across different populations. It is recommended to involve GP. Mur reagent cells in the antibody screening cells for populations with a high frequency of GYP Mur allele.