Precise detection of weak partial D type 15 in the Chinese population: evaluation of their potential impact on blood transfusion safety and development of appropriate response strategies
10.13303/j.cjbt.issn.1004-549x.2025.08.005
- VernacularTitle:中国人群弱部分D15型的精准检测及其对输血安全潜在影响的评估与应对策略
- Author:
Xu ZHANG
1
;
Zhuren ZHOU
1
;
Xuying HUANG
1
;
Lichun LI
1
;
Weiwei LI
1
;
Ping HOU
1
;
Xiaofeng LI
1
;
Jianping LI
1
Author Information
1. Institute of Transfusion Medicine, Shenyang Central Blood Station (Liaoning Blood Center), Shenyang 110044, China
- Publication Type:Journal Article
- Keywords:
weak partial D type 15;
RhD variant;
precise detection;
blood transfusion safety
- From:
Chinese Journal of Blood Transfusion
2025;38(8):1030-1034
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the precise detection methods for weak partial D type 15 and evaluate their implications for blood transfusion safety, along with the development of corresponding strategies. Methods: A combination of serological methods, including the microplate method, indirect antiglobulin tube method, and microcolumn gel card method, was employed to identify RhD-negative and RhD variant samples. RhD-negative samples were screened for the presence of RHD genes using whole-blood direct PCR amplification. Subsequently, RhD variant samples and RhD-negative samples containing RHD genes underwent full-coding-region sequencing of the RHD gene to confirm their genotypes. The genotyping results were further correlated with the serological test findings for comprehensive analysis. Results: Among 615 549 first-time healthy blood donors, 3 401 samples with an RhD-negative phenotype and 156 samples with RhD variant were identified. Of the 3 401 RhD-negative samples, 1 054 were found to harbor RHD genes. Gene sequencing analysis of the 156 RhD variants and the 1 054 serological negative samples revealed that 89 samples contained the RHD
15 (c. 845G>A) allele. Conclusion: The integration of serological testing methods and genotyping technologies for the precise determination of RhD blood type plays a critical role in ensuring the safety and compatibility of blood transfusions.
