Transcriptome Analysis of the Effects of Gomisin A on the Recovery of Carbon Tetrachloride-Induced Damage in Rat Liver.
10.5625/lar.2011.27.2.161
- Author:
Young Mi CHOI
1
;
In Soo CHOI
;
Sang Mong LEE
;
Dae Youn HWANG
;
Young Whan CHOI
;
Young Hoon PARK
Author Information
1. Department of Horticultural Bioscience, College of Natural Resource & Life Science, Pusan National University, Miryang, Republic of Korea. ypark@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Gomisin A;
carbon tetrachloride;
hepatic necrosis;
microarray;
transcriptome
- MeSH:
Animals;
Bile;
Carbon;
Carbon Tetrachloride;
Cell Cycle;
Cell Death;
Cholestasis;
Cyclooctanes;
Dioxoles;
Focal Adhesions;
Gene Expression;
Gene Expression Profiling;
Genome;
Lignans;
Liver;
Oligonucleotide Array Sequence Analysis;
Rats;
Transcriptome
- From:Laboratory Animal Research
2011;27(2):161-169
- CountryRepublic of Korea
- Language:English
-
Abstract:
Gomisin A possesses a hepatic function-facilitating property in liver-injured rats. Its preventive action on carbon tetrachloride-induced cholestasis is due to maintenance of the function of the bile acids-independent fraction. To investigate alterations in gene expression after gomisin A treatment on injured rat liver, DNA microarray analyses were performed on a Rat 44K 4-Plex Gene Expression platform with duplicated reactions after gomisin A treatment. We identified 255 up-regulated and 230 down-regulated genes due to the effects of gomisin A on recovery of carbon tetrachloride-induced rat liver damage. For functional characterization of these genes, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes biochemical pathways analyses were performed. Many up-regulated or down-regulated genes were related to cell cycle or focal adhesion and cell death genes, respectively. Our microarray experiment indicated that the liver repair mechanism induced by gomisin A was strongly associated with increased gene expressions related to cell cycle and suppression of the gene expression related in cell death.
