Characteristics of HIV primary drug resistance and molecular transmission clusters in newly reported men who had sex with men in Taizhou City， Zhejiang Province
10.19428/j.cnki.sjpm.2025.24830
- VernacularTitle:浙江省台州市新报告男男性行为人群人类免疫缺陷病毒原发耐药及分子传播特征
- Author:
Shanling WANG
1
;
Xuanhe WU
2
;
Guixia LI
1
;
Tingting WANG
1
;
Yating WANG
1
;
Tailin CHEN
2
;
Weiwei SHEN
1
;
Yali XIE
1
;
Haijiang LIN
1
;
Na HE
2
;
Xiaoxiao CHEN
3
Author Information
1. Taizhou Center for Disease Control and Prevention, Taizhou, Zhejiang 318000, China
2. School of Public Health, Key Laboratory of Public Health Safety of Ministry of Education, Fudan University, Shanghai 200032, China
3. Taizhou Blood Center, Taizhou, Zhejiang 318000, China
- Publication Type:Journal Article
- Keywords:
human immunodeficiency virus;
men who have sex with men;
recent infection;
molecular transmission network
- From:
Shanghai Journal of Preventive Medicine
2025;37(6):496-502
- CountryChina
- Language:Chinese
-
Abstract:
ObjectivesTo investigate the molecular epidemiological characteristics of HIV-1 infection among men who had sex with men (MSM) in Taizhou City, Zhejiang Province, and to provide a scientific reference for acquired immune deficiency syndrome prevention and control efforts. MethodsThe research subjects were all newly reported MSM population in Taizhou City from 2020 to 2023. Blood samples without antiviral therapy were collected. The HIV-1 pol gene was amplified and sequenced, and the sequences were submitted to the Stanford University drug resistance database to identify the mutation sites and drug resistance. MEGA 11.0 software was used to analyze the nucleic acid sequences, construct phylogenetic tree, and calculate genetic distance of gene sequences. The molecular transmission network diagram of HIV-1 was constructed using Cytoscape_v3.10.1, and the influencing factors of network entry were analyzed by logistic regression. ResultsA total of 363 newly reported HIV-infected MSM patients were included, with a median age [M (P25, P75)] of 34 (26,47) years old. The majority had an educational level of junior high school or below (55.65%). A total of eight subtypes were found, mainly CRF07_BC and CRF01_AE. The primary drug resistance rate was 10.47% (38/363). The optimal molecular network gene distance was 0.019, with a network access rate of 42.70% (155/363), and a total of 47 molecular clusters were formed. Multivariate logistic analyses showed that compared with the CRF01_AE subtype, the clustering risk of CRF07_BC subtype was higher (OR=1.916, 95%CI: 1.191‒3.109), cases with drug resistance had a higher risk of cluster formation than those without drug resistance (OR=2.011, 95%CI: 1.006‒4.080), and recent infected patients had a lower risk of entering the largest molecular cluster than long-term infected patients (OR=0.376, 95%CI: 0.137‒0.928). ConclusionThe newly diagnosed infections among the MSM population are active in Taizhou City, Zhejiang Province, with a high level of primary drug resistance. Individuals carrying drug-resistant strains are more likely to cluster. Drug resistance monitoring should be strengthened to prevent further spread of drug-resistant strains in the network.
