Effects and its mechanism of Jingui shenqi pill on diabetic nephropathy-related osteoporosis in rats
- VernacularTitle:金匮肾气丸对糖尿病肾病性骨质疏松大鼠的影响及机制
- Author:
Wenjun DING
1
;
Mingxia SHEN
2
;
Yongrui GAO
3
Author Information
1. Dept. of Nephrology，Gansu Academy of Traditional Chinese Medicine&Gansu Provincial Hospital of Traditional Chinese Medicine，Lanzhou 730050，China
2. Dept. of General Medicine，the Affiliated Hospital of Gansu University of Chinese Medicine，Lanzhou 730020，China
3. College of Integrated Traditional Chinese and Western Medicine，Gansu University of Chinese Medicine，Lanzhou 730020，China
- Publication Type:Journal Article
- Keywords:
Jingui shenqi pill;
diabetic nephropathy-
- From:
China Pharmacy
2025;36(16):2000-2004
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effect of Jinkui shenqi pill and its potential mechanism on diabetic nephropathy-related osteoporosis （DNOP） in rats. METHODS The rats were randomly divided into blank control group， control group， model group， Jingui shenqi pill low-， medium- and high-dose groups （0.62， 1.24， 2.48 g/kg）， and positive control group （denosumab 3 mg/ kg）， with 12 cases in each group. Except for the blank control group and the control group， models of DNOP were constructed in the remaining groups by high-glucose and high-fat forage， 3% fructose solution for feeding+intraperitoneal injection of streptozotocin solution. At 2 d after successful molding， Jingui shenqi pill low-， medium- and high-dose groups， and positive control group were given intragastric administration or subcutaneous injection of the corresponding drugs， once a day， for consecutive 8 weeks. After the last administration， bone mineral density （BMD） and serum bone metabolism-related indexes [osteocalcin （OC）， calcium， phosphorus， alkaline phosphatase （ALP）， tartrate-resistant acid phosphatase （TRAP）] were detected. The pathological changes of right femoral tissues were observed， and the expressions of receptor activator of NF-κB ligand （RANKL）/receptor activator of NF-κB （RANK）/osteoprotegerin （OPG） pathway-related mRNAs and proteins in left femoral tissues were detected. RESULTS Compared with the blank control group and the control group， there was rupture of bone trabecula and severe structural damage in the model group， and tissue space was obvious. BMD and ALP activities， mRNA and protein expressions of OPG were significantly decreased or down-regulated， while the levels of OC， calcium and phosphorus， TRAP activity， mRNA and protein expressions of RANKL and RANK were significantly increased or up-regulated （P＜0.05）. Compared with the model group， the number of bone trabeculae was increased in all Jingui shenqi pill groups， and the arrangement of bone trabeculae was more regular and dense. The above indexes generally were improved in a certain dose-dependent manner （P＜0.05）. CONCLUSIONS Jingui shenqi pill can promote osteogenesis and regulate the balance of bone metabolism in DNOP rats， which may be related to down-regulating the expressions of RANKL and RANK， and up-regulating the expression of OPG.
