Effects of Lishukang capsules on hypoxic pulmonary artery hypertension in rats by modulating the HIF-1α/NF-κB signaling pathway
- VernacularTitle:利舒康胶囊调节HIF-1α/NF-κB信号通路对大鼠低氧性肺动脉高压的影响
- Author:
Longyu LI
1
;
Fuju XU
1
;
Xin WANG
1
;
Songda ZHANG
2
Author Information
1. Dept. of Pharmacy，Qinghai Provincial People’s Hospital，Xining 810000，China
2. Dept. of Pharmacy，Fourth People’s Hospital of Qinghai Province，Xining 810000，China
- Publication Type:Journal Article
- Keywords:
Lishukang capsules;
hypoxic pulmonary artery hypertension;
pulmonary vascular remodeling;
HIF-1α;
NF-κB
- From:
China Pharmacy
2025;36(16):1988-1992
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effects of Lishukang capsules on hypoxic pulmonary artery hypertension （HPAH） rats by regulating the HIF-1α/NF-κB signaling pathway. METHODS Sixty rats were randomly divided into control group， model group， positive control group （sildenafil， 30 mg/kg）， Lishukang low-， medium- and high-dose groups （Lishukang capsules 6， 12， 18 g/kg）， with 10 rats in each group. Except for control group， the HPAH model was induced by intermittent hypoxia method （simulating an altitude of 5 000 m） in other groups； at the same time， they were given relevant medicine or normal saline intragastrically， for consecutive 28 days. Within 24 hours of the last administration， the mean pulmonary artery pressure （MPAP） and right ventricule hypertrophy index （RVHI） of rats were detected； pathological morphology of lung tissue was observed， and the expression of α-smooth muscle actin （α-SMA）， mRNA expressions of HIF-1α and NF-κB as well as protein expressions of HIF-1α and NF-κB p65 in lung tissue were determined. RESULTS Compared with model group， MPAP and RVHI in Lishukang medium- and high-dose groups and positive control group were all decreased significantly （P＜0.05）； the expression of α-SMA， mRNA expressions of HIF-1α and NF-κB， and protein expressions of HIF-1α and NF-κB p65 in lung tissue were all decreased significantly （P＜0.05）； additionally， pulmonary vascular remodeling was improved to varying degrees. CONCLUSIONS Lishukang capsules may protect HPAH rats， the mechanism of which may be associated with inhibiting the HIF-1α/NF- κB signaling pathway.
