Effects of Rhizosphere Organic Acids on Metabolism of Hairy Roots of Salvia miltiorrhiza
10.13422/j.cnki.syfjx.20241413
- VernacularTitle:根际有机酸环境模拟对丹参毛状根代谢的影响
- Author:
Xinyu FU
1
;
Wenying HAN
1
;
Jidong JU
1
;
Bingqian ZHOU
1
;
Guohong YANG
1
;
Xiao WANG
1
;
Lanping GUO
2
;
Wei LIU
1
Author Information
1. Key Laboratory for Applied Technology of Sophisticated Analytic Instrument of Shandong Province， Shandong Analytical and Test Center， Qilu University of Technology （Shandong Academy of Sciences）， Jinan 250014， China
2. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
- Publication Type:Journal Article
- Keywords:
Salvia miltiorrhiza;
hairy root;
continuous cropping obstacles;
rhizosphere organic acid;
effect on metabolism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(18):166-174
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of rhizosphere organic acids secreted by the roots of Salvia miltiorrhiza on continuous cropping obstacles. MethodsThe mixed solution of organic acids in the rhizosphere of S. miltiorrhiza in continuous cropping and rotation cropping was added to the hairy roots subcultured for 21 days， and samples were collected on days 0， 2， 4， 6， 8， and 10. The changes of biomass， effective components， primary metabolites， secondary metabolites， antioxidant enzymes， and hormones in hairy roots of S. miltiorrhiza were observed and determined. ResultsCompared with the rotation cropping group and the blank control group， the simulation of organic acid secretion from the roots of S. miltiorrhiza had a significant inhibitory effect on the growth of hairy roots and decreased the content of effective components as well as total sugar and total protein in primary metabolites. Compared with the blank control group， the rotation cropping group and the continuous cropping group showed total sugar and total protein content decreases of 33.9% and 5.1%， respectively. On the other hand， the secretion of organic acids from S. miltiorrhiza roots significantly promoted the accumulation of total phenolic acids and total tanshinone， which showed increases of 14.6% and 1.6%， respectively， in continuous cropping group and rotation cropping group compared with the blank control group. ConclusionThe organic acid environment under continuous cropping significantly inhibited the growth of hairy roots and the accumulation of primary metabolites， while promoting the synthesis and accumulation of secondary metabolites of S. miltiorrhiza.
