Quality Evaluation of Black Panacis Quinquefolii Radix Based on Neuroprotective Spectrum-effect Relationship
10.13422/j.cnki.syfjx.20251561
- VernacularTitle:基于神经元保护谱效关系的黑西洋参质量评价
- Author:
Yuting YANG
1
;
Shuyun LIANG
1
;
Shanshan LI
1
;
Yulong YANG
1
;
Ziqi YANG
1
;
Guangzhi CAI
1
;
Liru ZHAO
2
;
Jiyu GONG
1
Author Information
1. School of Pharmaceutical Sciences，Changchun University of Chinese Medicine，Changchun 130117，China
2. Jilin Institute of Biology，Changchun 130012，China
- Publication Type:Journal Article
- Keywords:
black Panacis Quinquefolii Radix;
neuroprotection;
spectrum-effect relationship;
content determination;
quality evaluation;
zebrafish;
ginsenoside
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(18):149-156
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo clarify the neuroprotective effect of black Panacis Quinquefolii Radix（PQR） and explore its active ingredients， with the aim of establishing an activity-oriented quality evaluation method. MethodsTransgenic Tg（HuC∶EGFP） zebrafish was used to establish a neuronal injury model by aluminum chloride immersion. Different doses（10， 20 mg·L-1） of PQR and black PQR ethanol extracts were administered. The neuroprotective effects of PQR and black PQR were compared by analyzing the fluorescent area and intensity of zebrafish neurons. Based on ultra-performance liquid chromatography（UPLC）， a fingerprint profile of black PQR was established， followed by principal component analysis（PCA） and orthogonal partial least squares-discriminant analysis（OPLS-DA）. Differential components were screened using the criteria of variable importance in the projection（VIP） value>1 and P<0.05. The neuroprotective activity of 14 batches of black PQR was assessed， and Spearman correlation analysis was used to identify saponins related to neuroprotective activity， which were then validated. Based on the above results， active marker components were determined， and an UPLC method was established for their quantitation with clear content limits. ResultsPharmacological efficacy results showed that both PQR and black PQR at different doses could significantly improved neuronal damage in zebrafish. At a dose of 20 mg·L-1， black PQR demonstrated superior efficacy（P<0.05）. The fingerprint similarities of 14 batches of black PQR were>0.94， with 26 common peaks identified. Through comparison with the reference standards， 8 components were confirmed， including peak 1（ginsenoside Rg1）， peak 2（ginsenoside Re）， peak 5（ginsenoside Rb1）， peak 9（ginsenoside Rd）， peak 16［ginsenoside 20（S）-Rg3］， peak 17［ginsenoside 20（R）-Rg3］， peak 18（ginsenoside Rk1）， and peak 19（ginsenoside Rg5）. The results of PCA and OPLS-DA indicated that there were differences in saponins among black PQR samples from different origins， and 12 differential components were screened. All 14 batches of black PQR exhibited good protective effects on zebrafish neurons， with Shaanxi-produced black PQR showing superior protective effects compared to the other three production regions. Spearman correlation analysis revealed that a total of 11 components， including ginsenosides 20（S）-Rg3， 20（R）-Rg3， Rk1 and Rg5， showed a significant positive correlation with the neuroprotective effect in zebrafish（P<0.05）. The activity validation results indicated that ginsenosides 20（S）-Rg3， 20（R）-Rg3， Rk1 and Rg5 were the primary components responsible for the neuroprotective effects of black PQR. Quantitative analysis showed that the content of ginsenoside 20（S）-Rg3 in 14 batches of black PQR ranged from 0.17% to 0.52%， and the repair rate of neuronal damage ranged from 42.77% to 97.83%. ConclusionBased on the fingerprint and neuronal protective activity， the spectrum-effect related quality control model of black PQR was established， with ginsenoside 20（S）-Rg3 as the quality control index， and the neuronal damage repair rate≥60% as the evaluation standard， the minimum limit of ginsenoside 20（S）-Rg3 in black PQR should be≥0.20%.
