Effect of Scutellariae Radix Combined with EGFR-TKIs on Non-small Cell Lung Cancer
10.13422/j.cnki.syfjx.20242127
- VernacularTitle:黄芩联合EGFR-TKIs对非小细胞肺癌的影响
- Author:
Yaya YU
1
;
Chenjing LEI
1
;
Zhenzhen XIAO
1
;
Qi MO
2
;
Changju MA
1
;
Lina DING
1
;
Yadong CHEN
1
;
Yanjuan ZHU
1
;
Haibo ZHANG
1
Author Information
1. The Second Clinical Medical School and Second Affiliated Hospital of Guangzhou University of Chinese Medicine， Guangzhou 510120， China
2. School of Traditional Chinese Medicine of Guangdong Pharmaceutical University， Guangzhou 510120， China
- Publication Type:Journal Article
- Keywords:
non-small cell lung cancer （NSCLC）;
Scutellariae Radix;
epidermal growth factor receptor-tyrosine kinase inhibitors （EGFR-TKIs）;
cancer stem cells;
reactive oxygen species （ROS）;
redox ratio
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(18):106-115
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of Scutellariae Radix combined with epidermal growth factor receptor-tyrosine kinase inhibitors （EGFR-TKIs） on cell proliferation， apoptosis， cancer stem cell （CSC） marker expression， and metabolism in non-small cell lung cancer （NSCLC） cells. MethodsThe anti-tumor effects of Scutellariae Radix and EGFR-TKIs （gefitinib or osimertinib） in NSCLC cells were evaluated using the cell counting kit-8 （CCK-8） and Annexin V-FITC/propidium iodide （PI） double staining apoptosis assay. The activity of Scutellariae Radix and EGFR-TKIs in three-dimensional （3D） cultures of NSCLC cells was assessed using the CellTiter-Glo® 3D cell viability assay. The mRNA and protein expression levels of CSC markers， sex determining region y box protein 2 （SOX2） and aldehyde dehydrogenase 1 family member A1 （ALDH1A1）， were detected by quantitative real-time polymerase chain reaction （Real-time PCR） and Western blot， respectively. Changes in intracellular reactive oxygen species （ROS） levels were detected by ROS staining， and the redox ratio was detected by femtosecond laser labeling free imaging （FLI）. ResultsUnder both two-dimensional （2D） and 3D culture conditions， compared with the blank group and EGFR-TKI group， the combination group showed significantly reduced cell viability and increased apoptosis rate （P<0.05）. Compared with the EGFR-TKI group， the mRNA and protein levels of CSC markers were significantly downregulated in the combination group （P<0.05）. Additionally， the redox ratio was significantly elevated （P<0.05）， and ROS levels were also increased in the combination group compared with the EGFR-TKI group. ConclusionIn NSCLC cells， Scutellariae Radix enhances the redox ratio and increases ROS levels， thereby inhibiting the expression of CSC markers and strengthening the anti-tumor effects of EGFR-TKIs. This provides a novel molecular mechanism by which Scutellariae Radix may enhance the sensitivity of targeted therapies.
