Preparation of vancomycin hydrochloride-loaded polydopamine nanoparticles and their antimicrobial effect in combination with photothermal therapy
- VernacularTitle:负载盐酸万古霉素的聚多巴胺纳米粒的制备及其联合光热的抗菌作用研究
- Author:
Lihua PAN
1
,
2
;
Bin LIU
3
;
Xiayun JIANG
2
;
Xiaopeng ZHOU
2
Author Information
1. School of Pharmacy，Zhejiang Chinese Medical University，Hangzhou 310053，China
2. Dept. of Pharmacy，Lishui People’s Hospital，Zhejiang Lishui 323000，China
3. Dept. of Orthopaedics，Lishui Municipal Central Hospital，Zhejiang Lishui 323000，China
- Publication Type:Journal Article
- Keywords:
vancomycin;
polydopamine;
nanoparticles;
photothermal therapy;
antibacterial;
biocompatibility
- From:
China Pharmacy
2025;36(15):1887-1892
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To prepare vancomycin hydrochloride （VH）-loaded polydopamine （PDA） nanoparticles （VH@PDA nanoparticles）， and study their antimicrobial effect in combination with photothermal therapy. METHODS Using PDA as the carrier， VH was loaded to prepare VH@PDA nanoparticles （PDA nanoparticles were prepared using the same method）. The nanoparticles were characterized with laser particle size analyzer， transmission electron microscope， and UV visible absorption spectrometer； the drug loading capacity and encapsulation efficiency of the nanoparticles were determined. The near-infrared laser irradiation was adopted to determine their photothermal ability. Taking Staphylococcus aureus as the research object， the bactericidal properties of the nanoparticles in combination with photothermal therapy in vitro were clarified through plate colony coating method， live/dead staining analysis， crystal violet staining. Using L929 cells as the research object， the effects of VH@PDA nanoparticles at different mass concentrations （0， 3.125， 6.25， 12.5， 25， 50 and 100 μg/mL） on cell viability were investigated to assess their biocompatibility. RESULTS VH@PDA nanoparticles were successfully loaded with VH， exhibiting uniform particle size （approximately 300 nm）， distinct pore size， and a spherical structure. The drug loading capacity was 11.34%， the encapsulation efficiency was 32.00%， the photothermal conversion efficiency reached 23.55%， and they demonstrated stable photothermal performance. The antibacterial effect results of the combined photothermal therapy demonstrated that without near- infrared laser irradiation， the antibacterial effects of VH， PDA nanoparticles， and VH@PDA nanoparticles were not significant. However， when subjected to near-infrared laser irradiation， VH@PDA nanoparticles exhibited a pronounced antibacterial effect. The results of the cell experiments revealed that after treatment with VH@PDA nanoparticles at various mass concentrations， the cell viability rates remained above 80%. CONCLUSIONS VH@PDA nanoparticles are successfully prepared， which exhibit stable photothermal properties， significant antibacterial effects when combined with photothermal therapy， and good biocompatibility.
