HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantation
10.13303/j.cjbt.issn.1004-549x.2025.07.017
- VernacularTitle:急性淋巴细胞白血病亲缘HLA半相合造血干细胞移植后的HLA分型及STR位点嵌合分析
- Author:
Zhanrou QUAN
1
;
Jie LIU
1
;
Bingna YANG
1
;
Yinming ZHANG
1
;
Hongyan ZOU
1
Author Information
1. Institute of Transfusion Medicine, Shenzhen Blood Center, Shenzhen 518040, China
- Publication Type:Journal Article
- Keywords:
HLA typing;
STR loci;
hematopoietic stem cell transplantation;
acute lymphoblastic leukemia;
chimerism
- From:
Chinese Journal of Blood Transfusion
2025;38(7):975-979
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the HLA typing and STR loci chimerism in a patient with recurrent acute lymphoblastic leukemia after HLA-haploidentical hematopoietic stem cell transplantation. Methods: HLA typing was performed on peripheral blood, buccal swabs and saliva samples after transplantation using PCR-sequence-specific oligonucleotide probes (PCR-SSOP) and next-generation sequencing (NGS). Additionally, STR analysis was conducted on these samples using a 21-locus STR assay kit to detect STR loci. Results: The HLA typing and STR locus outcomes of the patient's peripheral blood and the second saliva sample post-transplantation were in full concordance with the test results of the donor (father), whereas the HLA typing and STR locus results derived from the buccal swabs and the first saliva sample indicated chimerism between the donor and the recipient. Conclusion: In the follow-up and monitoring after transplantation, apart from focusing on peripheral blood samples, it is recommended to regularly monitor HLA typing and STR loci chimerism in patients' buccal swabs and saliva samples to comprehensively evaluate the transplantation effect and recurrence risk.
