Comparison of the efficacy of heat and acid elution methods for IgG anti-M and anti-Ku
10.13303/j.cjbt.issn.1004-549x.2025.07.015
- VernacularTitle:热放散和酸放散对IgG抗-M和抗-Ku放散效率比较
- Author:
Qunjuan ZENG
1
,
2
;
Huaiying KANG
3
;
Dong XIANG
4
;
Wei SHEN
4
;
Chengrui QIAN
4
;
Zhongying WANG
4
;
Guoqin GONG
4
Author Information
1. Dzhou Central Hospital, Dazhou 635000, China
2. Shanghai Blood Center, Shanghai 200051, China
3. Liupanshui People's Hospital, Liupanshui 553001, China
4. Shanghai Blood Center, Shanghai 200051, China
- Publication Type:Journal Article
- Keywords:
anti-M;
anti-Ku;
heat elution;
acid elution;
antibody titer
- From:
Chinese Journal of Blood Transfusion
2025;38(7):964-968
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the efficacy of heat and acid elution methods for IgG anti-M and anti-Ku. Methods: Ten samples with IgG anti-M and two samples with IgG anti-Ku were selected and standardized to a titer of 64. These antibodies underwent overnight absorption at 4℃ with O-type MM and kk-type erythrocytes, and then heat and acid elution methods were used on the absorbed sensitized erythrocytes respectively by detecting the titer of anti-M and anti-Ku in the eluate to compare the differences in the elution efficiency of IgG anti-M and anti-Ku between the two elution methods. Results: In heat elution tests, all 10 anti-M samples showed positive results with titers ranging from 8 to 64, while 2 anti-Ku samples yielded negative results. In acid elution tests, all 10 anti-M samples demonstrated negative results, whereas both anti-Ku (n=2) samples exhibited positive reactions with consistent titers of 32. Following acid elution with subsequent heat elution, 8 of 10 anti-M samples showed positive results with titers ranging from 8 to 32, while 2 remained negative. Both anti-Ku samples demonstrated positive with titers of 4. Conclusion: Heat elution demonstrated superior efficiency for IgG anti-M compared to acid elution, whereas acid elution showed greater efficacy for IgG anti-Ku than heat elution.
