Effect of nano hemoglobin-based oxygen carrier on radiosensitivity of lung cancer cell line

Changmin LIU; Yong LI; Fengjuan LI; Wentao ZHOU; Bingzhong HUANG; Jiaxin LIU; Chengmin YANG

Return

Effect of nano hemoglobin-based oxygen carrier on radiosensitivity of lung cancer cell line

VernacularTitle:纳米血红蛋白氧载体对肺癌细胞系放疗敏感性的影响
Author: Changmin LIU ^{1
,

2} ; Yong LI ^{1
,

2} ; Fengjuan LI ³ ; Wentao ZHOU ¹ ; Bingzhong HUANG ¹ ; Jiaxin LIU ¹ ; Chengmin YANG ^{1
,

2}
Author Information

1. Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu 610052, China
2. Tianjin Union Biotechnology Company l.t.d, Tianjin 300450, China
3. School of Pharmaceutical Sciences, Tiangong University, Tianjin 300387, China
Publication Type:Journal Article
Keywords: nano hemoglobin-based oxygen carrier (nano-HBOC); lung cancer; radiotherapy; oxygen sensitization
From: Chinese Journal of Blood Transfusion 2025;38(7):867-872
CountryChina
Language:Chinese
Abstract: Objective: To study the effect of nano hemoglobin-based oxygen carrier (nano-HBOC) on radiosensitivity of lung cancer H385 cells. Methods: Using 95% N and 5% CO , a lung cancer cell line was constructed in a hypoxic environment, and H385 cells were treated with different concentrations of nano-HBOC and irradiated (4Gy) by an irradiator, and the IC50 concentration was calculated. The cells were detected by flow cytometry (reactive oxygen species, ROS) ROS test. Using GEO database, KEGG pathway enrichment analysis was carried out to predict possible pathways. The levels of lipid peroxidation and Fe were observed by fluorescence microscope, and the proteins related to iron death pathway were detected by Western-blot. Results: Compared with the control cells, the activity and density of the cells were significantly decreased by nano-HBOC combined with radiotherapy, with a notable proportion of cells exhibiting deteriorated status. There is a positive correlation between ROS level and nano-HBOC concentration, especially after radiotherapy. Radiotherapy combined with nano-HBOC significantly increased the levels of lipid peroxidation and Fe in H385 cells, while decreasing the levels of iron death pathway proteins slc7a11 and GPX4, and increasing the level of ACSL4. Conclusion: Nano-HBOC enhances the radiosensitivity of lung cancer H385 cells.